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Dibenzylfluorescein

Catalog No. T78590 CAS 97744-44-0

Synonyms: DBF

Dibenzylfluorescein (DBF), a fluorogenic probe (Fluorescent dye), serves as a substrate for various cytochrome P450 (CYP) isoforms, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). It is commonly utilized at its K_m value range of 0.87-1.9 µM (Ex=485nm, Em=535nm) to monitor alterations in CYP enzymatic activity attributable to pharmaceutical interventions or pathophysiological conditions [1] [2] [3] [4].

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Dibenzylfluorescein, CAS 97744-44-0

Pack Size	Availability	Price/USD
1 mg	35 days	$ 110.00
5 mg	35 days	$ 430.00
10 mg	35 days	$ 786.00
25 mg	35 days	$ 1,730.00

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Biological Description

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Description

In vitro

P450-Catalyzed Metabolism of Dibenzylfluorescein and the Influence of Alkali Experimental Method [3]: During the reaction, P450 enzymes debenzylate Dibenzylfluorescein to yield fluorescent fluorescein benzyl ester, which can be hydrolyzed to fluorescein in the presence of sodium hydroxide. The addition of 2M sodium hydroxide also results in the decomposition of Dibenzylfluorescein into fluorescein benzyl ether. The conditions for CYP2C19 enzyme activity and inhibition experiments include a 150 μL reaction mixture containing 0.1M Tris-HCl buffer (pH 7.4), 10 μM Dibenzylfluorescein, 15 pmol CYP2C19, and 50 μL NADPH regenerating system. The NADPH regenerating system comprises 1.13 mM NADP, 12.5 mM citric acid, 56.33 mM KCl, 187.5 mM Tris-HCl (pH 7.4), 12.5 mM MgCl2, 0.0125 mM MnCl2, and 0.075 U/ml citrate dehydrogenase. Samples are incubated at 37°C for 30-60 minutes, rapidly cooled to 4°C to stop the reaction, centrifuged, and the supernatant is analyzed by LC-MS. Analyses are carried out using pure Dibenzylfluorescein, fluorescein benzyl ester, fluorescein benzyl ether, and fluorescein (all at 10 μM) as standards, both in the absence and presence of 2 M NaOH.

Synonyms	DBF
Molecular Weight	512.55
Formula	C34H24O5
CAS No.	97744-44-0

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Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Dibenzylfluorescein 97744-44-0 DBF inhibitor inhibit

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