β-Elemene (Levo-β-elemene) is a natural product isolated from Curcuma wenyujin, with an antitumor activity and induce cell apoptosis.

β-Elemene significantly inhibited the metastatic capacity of MDR gastric cells in vivo and in vitro.?Mechanistically, β-Elemene regulated MMP-2/9 expression and reversed epithelial-mesenchymal transition.?β-Elemene upregulated Cbl-b expression, resulting in inhibition of the EGFR-ERK/AKT pathways, which regulate MMP-2/9.?β-Elemene upregulated Cbl-b by inhibiting miR-1323 expression.?Numbers of metastatic tumor nodules were significantly decreased in the lungs of nude mice after β-Elemene treatment.?β-Elemene inhibits the metastasis of MDR gastric cancer cells by modulating the miR-1323/Cbl-b/EGFR signaling axis[3].

Cell viability assay was used to assess the resistance of SGC7901/ADR cells and the cytotoxic effects of β-Elemene.?Wound healing, transwell assay and lung metastatic mice model were used to the anti-metastasis effects of β-Elemene.?MicroRNA microarray analysis was used to explore potential regulated miRNAs.?Luciferase reporter assay was used to identify the direct target.?Human MMP antibody array, western blot, immunoprecipitation, qRT-PCR analyses and immunohistochemistry were conducted to investigate the underlying anti-metastasis mechanism of β-Elemene[3].