Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow)

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow)

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Heart (Mouse) Lysate at 40 μg
Lane 3: Cerebellum (Mouse) Lysate at 40 μg
Lane 4: Testis (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Lane 7: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Oct4 (TMAB-01562) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-4-Oct antibody (TMAB-01562)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control (Black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-Oct4 antibody (TMAB-01562)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human OCT4

Human

5460

DNA / RNA binding,POU,Transcription Factors