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Anti-POU5F1 Polyclonal Antibody

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Catalog No. TMAB-01562
Alias transcription factor 1, POU5F1, POU domain, class 5, transcription factor 1, OTF3, Octamer-binding transcription factor 3 (OTF-3), Octamer-binding protein 4 (Oct-4), Octamer-binding protein 3 (Oct-3), class 5

Anti-POU5F1 Polyclonal Antibody is a Rabbit antibody targeting POU5F1. Anti-POU5F1 Polyclonal Antibody can be used in FCM,WB.

Anti-POU5F1 Polyclonal Antibody

Anti-POU5F1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01562Alias transcription factor 1, POU5F1, POU domain, class 5, transcription factor 1, OTF3, Octamer-binding transcription factor 3 (OTF-3), Octamer-binding protein 4 (Oct-4), Octamer-binding protein 3 (Oct-3), class 5
Anti-POU5F1 Polyclonal Antibody is a Rabbit antibody targeting POU5F1. Anti-POU5F1 Polyclonal Antibody can be used in FCM,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-POU5F1 Polyclonal Antibody is a Rabbit antibody targeting POU5F1. Anti-POU5F1 Polyclonal Antibody can be used in FCM,WB.
Synonymstranscription factor 1, POU5F1, POU domain, class 5, transcription factor 1, OTF3, Octamer-binding transcription factor 3 (OTF-3), Octamer-binding protein 4 (Oct-4), Octamer-binding protein 3 (Oct-3), class 5
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow)
Verified Activity
1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Heart (Mouse) Lysate at 40 μg
Lane 3: Cerebellum (Mouse) Lysate at 40 μg
Lane 4: Testis (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Lane 7: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Oct4 (TMAB-01562) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-4-Oct antibody (TMAB-01562)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control (Black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-Oct4 antibody (TMAB-01562)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
Recommended Dose
WB: 1:500-2000; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.Note=Expressed in a diffuse and slightly punctuate pattern.
Tissue SpecificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human OCT4
Antigen Species
Human
Gene Name
POU5F1
Gene ID
Protein Name
POU domain, class 5, transcription factor 1
Uniprot ID
Biology Area
DNA / RNA binding,POU,Transcription Factors
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Chemical Properties
Molecular WeightTheoretical: 39 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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