Anti-POU5F1 Polyclonal Antibody

Anti-POU5F1 Polyclonal Antibody is a Rabbit antibody targeting POU5F1. Anti-POU5F1 Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow)

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Heart (Mouse) Lysate at 40 μg
Lane 3: Cerebellum (Mouse) Lysate at 40 μg
Lane 4: Testis (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Lane 7: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Oct4 (TMAB-01562) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-4-Oct antibody (TMAB-01562)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control (Black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-Oct4 antibody (TMAB-01562)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human OCT4

Human

POU5F1

POU domain, class 5, transcription factor 1

DNA / RNA binding,POU,Transcription Factors

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.