Rifampicin (Rifamycin AMP) is a broad-spectrum antibiotic. Rifampicin has a strong antibacterial effect against Mycobacterium tuberculosis and is also effective against gram-positive and gram-negative bacteria and viruses.

OATP1B1:1.2–1.5 μM, Vero cells:> 10 μg/mL, Osteoblast:130 μg/mL, HepG2 cells:145.33 μM, MDCK cells-II:> 20 μM, J774 cells.A1:> 64 μg/mL, THP-1 cells:0.0018 μg/mL, Hepatocyte cells:> 20 μM, MG-63 cells:240 μg/mL, PBMC cells:62.92 μg/mL, Macrophages:> 100 μM, MRC5 cells:> 100 μM, HeLa cells:270 μg/mL, Huh7 cells:> 20 μM, HEK293 cells:0.3 μM

METHODS: PC12 cells were treated with Rifampicin (25-100 µM) for 3-24 h. The expression levels of target proteins were detected by Western Blot.
RESULTS: At 6 h of incubation, Rifampicin induced a significant increase in GRP78 protein expression in PC12 cells. Prolonged incubation with Rifampicin further enhanced the induction of GRP78 up to 24 h. PC12 cells showed a dose-dependent induction of GRP78 when incubated with Rifampicin at concentrations in the 25-100 µM range. [1]
METHODS: Human colon cancer cells LS180 were treated with Rifampicin (10 µM) for 24-144 h. Gene levels were measured by RT-PCR.
RESULTS: The total expression of NR1I2 mRNA was reduced by 40% after 24 h of Rifampicin treatment, and the expression of PXR.3 mRNA was reduced by 45% after 24 h of Rifampicin treatment. [2]

Rifampicin (200, 400 mg/kg) can induce fatty liver at high concentrations [4]. Rifampicin (30 mg/kg, i.p.) treatment of S464P biofilms in vivo causes a slight decline in bioluminescence from these catheters compared with the parental signal, but it does not affect bioluminescence in mice infected with mutant H481Y [5].

DMSO: 87.5 mg/mL (106.33 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (6.08 mM), Solution.