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Columbianadin

Name: Columbianadin
Brand: TargetMol
SKU: T3373
Price: 60 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3373Cas No. 5058-13-9

Alias Zosimin, 2-Butenoic acid

Columbianadin (Zosimin), one of the main bioactive constituents of the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan, has been found to possess obvious pharmacological effects in previous studies.

Columbianadin

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Purity: 99.48%

Catalog No. T3373Alias Zosimin, 2-Butenoic acidCas No. 5058-13-9

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$60	In Stock	In Stock
20 mg	$100	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$60	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.48%

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Columbianadin (Zosimin), one of the main bioactive constituents of the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan, has been found to possess obvious pharmacological effects in previous studies.
Kinase Assay	Cells are treated with various concentrations of Dihydrotanshinone I (3.13-20 μM) for 48 h. For the activity assay, Ac-DEVD-AMC (1 μg/μL), Ac-IETD-AMC (1 μg/μL) or Ac-LEDH-AMC (1 μg/μL) and cell lysate are added into Protease Assay Buffer in 96-well plate. Reaction mixtures with lysis buffer are used as negative controls. Cells treated with DMSO (0.1%) are treated as vehicle control. The reaction mixtures are incubated for 1 h at 37°C. The AMC liberated from the substrates is measured using spectrofluorometer of Victor 2 plate reader with an excitation wavelength of 380 nm and an emission wavelength of 430 nm.
Synonyms	Zosimin, 2-Butenoic acid

Chemical Properties

Molecular Weight	328.36
Formula	C₁₉H₂₀O₅
Cas No.	5058-13-9
Smiles	C\C=C(\C)C(=O)OC(C)(C)[C@@H]1Cc2c(O1)ccc1ccc(=O)oc21
Relative Density.	1.232 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Chloroform: Soluble

DMSO: 59 mg/mL (179.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (10.05 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0454 mL	15.2272 mL	30.4544 mL	152.2719 mL
5 mM	0.6091 mL	3.0454 mL	6.0909 mL	30.4544 mL
10 mM	0.3045 mL	1.5227 mL	3.0454 mL	15.2272 mL
20 mM	0.1523 mL	0.7614 mL	1.5227 mL	7.6136 mL
50 mM	0.0609 mL	0.3045 mL	0.6091 mL	3.0454 mL
100 mM	0.0305 mL	0.1523 mL	0.3045 mL	1.5227 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc