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Anti-CD68 Antibody (8F211)

😃Good
Catalog No. TMAB-00380
Alias SCARD1, LAMP4, GP110, CD68 molecule

Anti-CD68 Antibody (8F211) is a Mouse antibody targeting CD68. Anti-CD68 Antibody (8F211) can be used in IHC-P,IHC-Fr,IF.

Anti-CD68 Antibody (8F211)

Anti-CD68 Antibody (8F211)

😃Good
Catalog No. TMAB-00380Alias SCARD1, LAMP4, GP110, CD68 molecule
Anti-CD68 Antibody (8F211) is a Mouse antibody targeting CD68. Anti-CD68 Antibody (8F211) can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
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Product Introduction

Bioactivity
Description
Anti-CD68 Antibody (8F211) is a Mouse antibody targeting CD68. Anti-CD68 Antibody (8F211) can be used in IHC-P,IHC-Fr,IF.
AliasSCARD1, LAMP4, GP110, CD68 molecule
Ig Type
IgG
Clone
8F211
Reactivity
Human,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human diffuse large B-cell lymphoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD68) Monoclonal Antibody, Unconjugated (TMAB-00380) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-300; IHC-Fr: 1:100-300; IF: 1:100-300
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationIsoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
Recombinant Protein: human CD68 1-100/354aa
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Myeloid Cells,SARS Coronavirus
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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