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Acetaminophen

Acetaminophen
Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.
Catalog No. T0065Cas No. 103-90-2
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Acetaminophen

Catalog No. T0065Cas No. 103-90-2

Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.
Targets&IC50
COX-2:5.8 μM
In vitro
METHODS: Non-melanoma and melanoma cell lines were treated with Acetaminophen (100 µM) for 48 h and cell viability was measured using MTT Assay.
RESULTS: Acetaminophen showed considerable toxicity in melanoma cell lines SK-MEL-5, MeWo, B16-F0 and B16-F10, resulting in 40±3%, 45±7%, 66±8% and 60±5% cytotoxicity, respectively. Acetaminophen showed negligible toxicity at 100 μM in the non-melanoma cell lines PC-3, BJ, Saos-2 and SW-620 cells. [1]
METHODS: Neuroblastoma cells SH-SY5Y were treated with Acetaminophen (2 mM) for 24-48 h, and the expression levels of target proteins were detected using Western Blot.
RESULTS: Acetaminophen induced cytochrome c release from mitochondria in a time-dependent manner, reaching a maximum level after 48 h of treatment. In addition, immunoblot analysis of cytoplasmic and mitochondrial fractions showed that Acetaminophen was able to induce the accumulation of Bax into mitochondria at 24 h after treatment. [2]
In vivo
METHODS: To detect hepatotoxicity in vivo, Acetaminophen was administered intraperitoneally to mice (300 mg/kg) and rats (1 g/kg).
RESULTS: Extensive liver necrosis was observed in mice, but little damage was observed in rat samples. The rats were highly resistant to Acetaminophen-induced liver injury. [3]
METHODS: To test for in vivo hepatotoxicity, Acetaminophen was administered to aged and weakened mice acutely (300 mg/kg by gavage), chronically (100 mg/kg by diet once daily for six weeks), or subacutely (250 mg/kg by gavage three times daily for three days).
RESULTS: There was no overall increase in Acetaminophen hepatotoxicity with age or frailty in mice, despite changes in certain pathways that would be expected to affect susceptibility to Acetaminophen toxicity. [4]
Kinase Assay
Effect of inhibition of Acetaminophen on COX-1 and COX-2 activity in human whole blood: For COX-1 assay, aliquots of human whole blood drawn from healthy volunteers without anticoagulant are transferred to glass tubes containing Acetaminophen or DMSO, serum is separated by centrifugation after clotting, and serum TxB2 levels are determined. For COX-2 assay, aliquots of heparinized whole blood are incubated with LPS (10 μg/mL) and aspirin (10 μg/mL), plus Acetaminophen or DMSO for 24 hours at 37 °C, plasma is separated by centrifugation, and PGE2 levels are determined subsequently. The degree of COX-1 or COX-2 inhibition is calculated as the percentage change of plasma eicosanoid (TxB2 for COX-1 and PGE2 for COX-2).Concentration response curves are fitted by a sigmoidal regression with variable slope for both enzymatic assays, and the 50% inhibitory concentration (IC50) values are derived by using of PRISM Version 3.0.
Cell Research
Cells are exposed to Acetaminophen for 48 hours. Cell viability is determined by the trypan blue exclusion method. Intracellular GSH is measured by recording the disulfide, GS-TNB and 5-thio-nitrobenzoic acid (TNB), the yellow colored compound formed by the reaction between GSH with DTNB.(Only for Reference)
Alias4-Acetamidophenol, 4'-Hydroxyacetanilide, APAP, Paracetamol
Chemical Properties
Molecular Weight151.16
FormulaC8H9NO2
Cas No.103-90-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 60 mg/mL (396.93 mM)
H2O: 20 mg/mL ( 132.3 mM), Sonication is recommended.
Ethanol: 15.1 mg/mL (100 mM)
Solution Preparation Table
Ethanol/H2O/DMSO
1mg5mg10mg50mg
1 mM6.6155 mL33.0775 mL66.1551 mL330.7753 mL
5 mM1.3231 mL6.6155 mL13.2310 mL66.1551 mL
10 mM0.6616 mL3.3078 mL6.6155 mL33.0775 mL
20 mM0.3308 mL1.6539 mL3.3078 mL16.5388 mL
50 mM0.1323 mL0.6616 mL1.3231 mL6.6155 mL
100 mM0.0662 mL0.3308 mL0.6616 mL3.3078 mL

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