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1-Pyrenebutyric acid NHS ester
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Catalog No. T66661Cas No. 114932-60-4
Alias 1-Pyrenebutyric acid N-hydroxysuccinimide ester
1-Pyrenebutyric acid NHS ester is an aromatic reagent widely used for biomolecule labeling. the NHS ester of 1-Pyrenebutyric acid NHS ester functionality enables covalent conjugation to amine-containing substrates, supporting applications in nucleic acid detection, protein immobilization, and characterization of cyclodextrin-based polyrotaxanes. Its pyrene fluorophore makes it suitable for fluorescence-based analytical studies.
1-Pyrenebutyric acid NHS ester
😃Good
Purity: 99.62%
Catalog No. T66661Alias 1-Pyrenebutyric acid N-hydroxysuccinimide esterCas No. 114932-60-4
1-Pyrenebutyric acid NHS ester is an aromatic reagent widely used for biomolecule labeling. the NHS ester of 1-Pyrenebutyric acid NHS ester functionality enables covalent conjugation to amine-containing substrates, supporting applications in nucleic acid detection, protein immobilization, and characterization of cyclodextrin-based polyrotaxanes. Its pyrene fluorophore makes it suitable for fluorescence-based analytical studies.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 mg | $35 | - | In Stock | |
| 500 mg | $68 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.62%
Appearance:Solid
Color:Yellow
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | 1-Pyrenebutyric acid NHS ester is an aromatic reagent widely used for biomolecule labeling. the NHS ester of 1-Pyrenebutyric acid NHS ester functionality enables covalent conjugation to amine-containing substrates, supporting applications in nucleic acid detection, protein immobilization, and characterization of cyclodextrin-based polyrotaxanes. Its pyrene fluorophore makes it suitable for fluorescence-based analytical studies. |
| In vitro | In in vitro experiments, antibodies were immobilized on the surface of CNT-FET circuits via a 1-hour reaction with 1-Pyrenebutyric acid NHS ester at a concentration of 5 mM [3]. 1-Pyrenebutyric acid NHS ester was employed as an interfacial coupling reagent, which enabled the efficient immobilization of SARS-CoV-2 spike antibodies on the surface of graphene sheets. This immobilization strategy provided critical support for the construction of field-effect transistor (FET)-based biosensors, which can be used for the specific detection of SARS-CoV-2 [5]. |
| Synonyms | 1-Pyrenebutyric acid N-hydroxysuccinimide ester |
| Molecular Weight | 385.42 |
| Formula | C24H19NO4 |
| Cas No. | 114932-60-4 |
| Smiles | O=C(ON1C(=O)CCC1=O)CCCC2=CC=C3C=CC4=CC=CC=5C=CC2=C3C45 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility Information | DMSO: ≥ 80 mg/mL, Sonication is recommended. |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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