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Anti-CXCR4 Polyclonal Antibody 2
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Catalog No. TMAB-00510
Alias WHIMS, WHIM, NPYY3R, NPYRL, NPYR, NPY3R, LESTR, LCR1, LAP-3, LAP3, HSY3RR, HM89, FB22, D2S201E, chemokine (C-X-C motif) receptor 4, CD184
Anti-CXCR4 Polyclonal Antibody 2 is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CXCR4 Polyclonal Antibody 2
Anti-CXCR4 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00510Alias WHIMS, WHIM, NPYY3R, NPYRL, NPYR, NPY3R, LESTR, LCR1, LAP-3, LAP3, HSY3RR, HM89, FB22, D2S201E, chemokine (C-X-C motif) receptor 4, CD184
Anti-CXCR4 Polyclonal Antibody 2 is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CXCR4 Polyclonal Antibody 2 is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | WHIMS, WHIM, NPYY3R, NPYRL, NPYR, NPY3R, LESTR, LCR1, LAP-3, LAP3, HSY3RR, HM89, FB22, D2S201E, chemokine (C-X-C motif) receptor 4, CD184 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Blood cell (Mouse) Lysate at 40 μg Lane 2: LympHnode (Mouse) Lysate at 40 μg Lane 3: Bone (Rat) Lysate at 40 μg Lane 4: Spleen (Rat) Lysate at 40 μg Primary: Anti-CXCR4 (TMAB-00510) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 44 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CXCR4) Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CXCR4) Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 5. Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 6. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 7. Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 8. Paraformaldehyde-fixed, paraffin embedded Human prostate tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 9. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 10. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 11. Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00510) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Monomer. Can form dimers. |
| Immunogen | KLH conjugated synthetic peptide: human CXCR4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Regulatory T Cells,HIV-related,Alpha Chemokine Rec. (CXCR),Hypoxia,Host Virus Interaction,SARS Coronavirus,Axonal Guidance Proteins,Endothelial Markers,Surface Molecules,Surface Molecules |
| Molecular Weight | Theoretical: 40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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