CYP2C9 Protein, Human, Recombinant (His & Myc) is expressed in E. coli.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 20 days | $ 284.00 | |
100 μg | 20 days | $ 537.00 | |
1 mg | 20 days | $ 2,300.00 |
Description | CYP2C9 Protein, Human, Recombinant (His & Myc) is expressed in E. coli. |
Species | Human |
Expression System | E. coli |
Tag | N-terminal 10xHis-tagged and C-terminal Myc-tagged |
Accession Number | P11712 |
Synonyms | CYP2C10, Cytochrome P450 2C9, S-mephenytoin 4-hydroxylase, (R)-limonene 6-monooxygenase, Cytochrome P450 MP-8, Cytochrome P450 MP-4, CYPIIC9, CYP2C9, Cytochrome P-450MP, (S)-limonene 7-monooxygenase, (S)-limonene 6-monooxygenase, Cytochrome P450 PB-1, Cholesterol 25-hydroxylase |
Amino Acid | MDSLVVLVLCLSCLLLLSLWRQSSGRGKLPPGPTPLPVIGNILQIGIKDISKSLTNLSKVYGPVFTLYFGLKPIVVLHGYEAVKEALIDLGEEFSGRGIFPLAERANRGFGIVFSNGKKWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKGG Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Construction | 1-162 aa |
Protein Purity | > 85% as determined by SDS-PAGE. |
Molecular Weight | 25.4 kDa (predicted) |
Formulation | Tris-based buffer,50% glycerol |
Reconstitution | A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2. Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol. Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CYP2C9 Protein, Human, Recombinant (His & Myc) CYP2C10 Cytochrome P450 2C9 S-mephenytoin 4-hydroxylase (R)-limonene 6-monooxygenase Cytochrome P450 MP-8 Cytochrome P450 MP-4 CYPIIC9 CYP2C9 Cytochrome P-450MP (S)-limonene 7-monooxygenase (S)-limonene 6-monooxygenase Cytochrome P450 PB-1 Cholesterol 25-hydroxylase recombinant recombinant-proteins proteins protein