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Robinin

Name: Robinin
Brand: TargetMol
SKU: T2S0265
Price: 397 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: NSC 9222) Copy Product Info

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Synonyms: NSC 9222

Catalog No. T2S0265 Copy Product Info

Purity: 99.96%

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Robinin (NSC-9222) belongs to natural products, serving as a TLR2 inhibitor with cell permeability. Robinin exerts anti-inflammatory and antitumor activities through inhibiting the TLR2-PI3K-AKT pathway, and can be used for experimental research related to pancreatic cancer.

Cas No. 301-19-9

Pack Size	Price	USA Stock	Global Stock
1 mg	$68	In Stock	In Stock
5 mg	$163	In Stock	In Stock
10 mg	$243	In Stock	In Stock
25 mg	$397	In Stock	In Stock
50 mg	Preferential	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:99.96%

Appearance:Solid

Color:White to Yellow

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Robinin (NSC-9222) belongs to natural products, serving as a TLR2 inhibitor with cell permeability. Robinin exerts anti-inflammatory and antitumor activities through inhibiting the TLR2-PI3K-AKT pathway, and can be used for experimental research related to pancreatic cancer.
Targets & IC50	A549 cells viability:34.49 μM
In vitro	Methods: Mouse primary chondrocytes were used. After 12 h treatment with IL-1β (10 ng/mL) to establish an injury model, cells were incubated with 10 μg/mL Robinin for 24 h. Results: CCK-8 and flow cytometry showed that Robinin reversed the IL-1β-induced decrease in cell viability and inhibited apoptosis; Western blot further confirmed its protective effects by upregulating Bcl-2 and downregulating Bax and cleaved-caspase3 expression. [1] Methods: H9c2 rat cardiomyocytes were used. Cells were pre-treated with 50 μM Robinin for 24 h, followed by addition of 40 μM H₂O₂ or 5 μM doxorubicin to induce injury. Results: MTT, AO/EB, and Hoechst staining showed that Robinin increased cell viability and reduced apoptosis; DCFH-DA and flow cytometry demonstrated its reduction of reactive oxygen species levels, enhancement of antioxidant enzyme activity, and inhibition of endoplasmic reticulum stress-related gene and protein expression. [2]
In vivo	Methods: In a hypercholesterolemic rat model, 2% cholesterol diet was used to induce hypercholesterolemia for 8 weeks. Starting from week 6, Robinin (50 mg/kg, once every two days, dissolved in DMSO; control received vehicle) was administered by intraperitoneal injection for 2 consecutive weeks, followed by ex vivo cardiac ischemia-reperfusion injury. Results: Robinin improved cardiac function, inhibited mitochondrial permeability transition pore opening, attenuated myocardial histopathological damage, upregulated myocardial Akt, Nrf2, HO-1, and NQO1 expression, and downregulated GSK3β and Fyn levels.[3]
Synonyms	NSC 9222

Chemical Properties

Molecular Weight	740.66
Formula	C₃₃H₄₀O₁₉
Cas No.	301-19-9
Smiles	C[C@@H]1O[C@@H](OC[C@H]2O[C@@H](Oc3c(oc4cc(O[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)cc(O)c4c3=O)-c3ccc(O)cc3)[C@H](O)[C@@H](O)[C@H]2O)[C@H](O)[C@H](O)[C@H]1O
Relative Density.	1.74 g/cm3

Storage & Solubility Information

Storage

Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Pyridine, Methanol, etc.: Soluble

Ethanol: Soluble

DMSO: 100 mg/mL (135.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (4.46 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3501 mL	6.7507 mL	13.5015 mL	67.5074 mL
5 mM	0.2700 mL	1.3501 mL	2.7003 mL	13.5015 mL
10 mM	0.1350 mL	0.6751 mL	1.3501 mL	6.7507 mL
20 mM	0.0675 mL	0.3375 mL	0.6751 mL	3.3754 mL
50 mM	0.0270 mL	0.1350 mL	0.2700 mL	1.3501 mL
100 mM	0.0135 mL	0.0675 mL	0.1350 mL	0.6751 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.