Anti-NR1H4 Polyclonal Antibody is a Rabbit antibody targeting NR1H4. Anti-NR1H4 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep)

1. Blank control: A549. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (TMAB-01264)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 3 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Pancreas tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human MCF-7 cell lysates
Primary: Anti-NR1H4 (TMAB-01264) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 50 kDa
3. Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (TMAB-01264)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2ug/test

Polyclonal

KLH conjugated synthetic peptide: human FXR/Bile Acid Receptor NR1H4

Human

Orphan Nuclear Receptors,Energy Metabolism,Energy Metabolism,Lipid metabolism