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Anti-APAF1(NT) Polyclonal Antibody

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Catalog No. TMAB-00126
Alias KIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF

Anti-APAF1(NT) Polyclonal Antibody is a Rabbit antibody targeting APAF1(NT). Anti-APAF1(NT) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-APAF1(NT) Polyclonal Antibody

Anti-APAF1(NT) Polyclonal Antibody

😃Good
Catalog No. TMAB-00126Alias KIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF
Anti-APAF1(NT) Polyclonal Antibody is a Rabbit antibody targeting APAF1(NT). Anti-APAF1(NT) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-APAF1(NT) Polyclonal Antibody is a Rabbit antibody targeting APAF1(NT). Anti-APAF1(NT) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasKIAA0413, CED4, CED 4, Apoptotic protease-activating factor 1, Apoptotic protease activating factor, Apoptotic peptidase activating factor 1, APAF1, APAF 1, APAF
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse)
Verified Activity
1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APAF1 (NT) Polyclonal Antibody, Unconjugated (TMAB-00126) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-APAF1 (NT) Polyclonal Antibody, Unconjugated (TMAB-00126) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Lung (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-APAF1 (NT) (TMAB-00126) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APAF1 (NT)) Polyclonal Antibody, Unconjugated (TMAB-00126) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (APAF1 (NT)) Polyclonal Antibody, Unconjugated (TMAB-00126) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (APAF1 (NT)) polyclonal Antibody, Unconjugated (TMAB-00126) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (APAF1 (NT)) polyclonal Antibody, Unconjugated (TMAB-00126) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
8. U-937 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with APAF1 (NT) Antibody (TMAB-00126) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 0.2ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain(NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Apaf-1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cytochrome C,Metabolism,Mitochondrial,Cytochrome C,Mitochondrial,Apoptosis,Mitochondrial markers
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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