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Anti-Phospho-MST1 (Thr183) Polyclonal Antibody

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Catalog No. TMAB-01456

Anti-Phospho-MST1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MST1 (Thr183). Anti-Phospho-MST1 (Thr183) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Phospho-MST1 (Thr183) Polyclonal Antibody

Anti-Phospho-MST1 (Thr183) Polyclonal Antibody

😃Good
Catalog No. TMAB-01456
Anti-Phospho-MST1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MST1 (Thr183). Anti-Phospho-MST1 (Thr183) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-MST1 (Thr183) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MST1 (Thr183). Anti-Phospho-MST1 (Thr183) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MST1 (Thr183)) Polyclonal Antibody, Unconjugated (TMAB-01456) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-MST1 (Thr183)) polyclonal Antibody, Unconjugated (TMAB-01456) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-MST1 (Thr183) antibody (TMAB-01456)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human Mst1 around the phosphorylation site of Thr183
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Apoptosis,Hypertrophy,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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