Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-CDKN2A Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00412
Anti-CDKN2A Polyclonal Antibody 2 is a Rabbit antibody targeting CDKN2A. Anti-CDKN2A Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CDKN2A Polyclonal Antibody 2
Anti-CDKN2A Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00412
Anti-CDKN2A Polyclonal Antibody 2 is a Rabbit antibody targeting CDKN2A. Anti-CDKN2A Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Pig) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDKN2A) Polyclonal Antibody, Unconjugated (TMAB-00412) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDKN2A) Polyclonal Antibody, Unconjugated (TMAB-00412) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: U937 (Human) Cell Lysate at 30 μg Primary: Anti-CDKN2A/p16-INK4a (TMAB-00412) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDKN2A/p16-INK4a) Polyclonal Antibody, Unconjugated (TMAB-00412) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]. |
| Immunogen | KLH conjugated synthetic peptide: human CDKN2A/p16-INK4a |
| Antigen Species | Human |
| Gene ID | 1029 |
| Uniprot ID | |
| Biology Area | Ink4,p53 pathway,Ink4,Ink4,Tumor Suppressors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-CDKN2A Polyclonal Antibody 2 | purchase Anti-CDKN2A Polyclonal Antibody 2 | Anti-CDKN2A Polyclonal Antibody 2 cost | order Anti-CDKN2A Polyclonal Antibody 2
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.