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Anti-MST1 Antibody (5S102)
😃Good
Catalog No. TMAY-01889
Alias YSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2
Anti-MST1 Antibody (5S102) is a Mouse antibody targeting MST1. Anti-MST1 Antibody (5S102) can be used in FCM,ICC/IF.
Anti-MST1 Antibody (5S102)
Anti-MST1 Antibody (5S102)
😃Good
Catalog No. TMAY-01889Alias YSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2
Anti-MST1 Antibody (5S102) is a Mouse antibody targeting MST1. Anti-MST1 Antibody (5S102) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | YSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2 |
| Ig Type | Mouse IgG1 |
| Clone | 5S102 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human STK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human STK4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm. 2. Flow cytometric analysis of Human STK4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human STK4 / MST1 (rh STK4 / MST1; TMPY-04398; Q13043-1; Glu2-Phe487). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Dysregulation of MST1/STK4, a key kinase component of the Hippo-YAP pathway, is linked to the etiology of many cancers with poor prognosis. STK4/Hippo pathway may have important therapeutic implications for cancer. The tumor suppressor serine/threonine-protein kinase 4 (STK4) differentially regulates TLR3/4/9-mediated inflammatory responses in macrophages and thereby is protective against chronic inflammation-associated Hepatocellular carcinoma (HCC). STK4 has potential as a diagnostic biomarker and therapeutic target for inflammation-induced HCC. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human STK4 / MST1 protein (TMPY-04398) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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