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Anti-FUT4 Polyclonal Antibody

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Catalog No. TMAB-00722
Alias SSEA-1, SSEA1, LeX, Galactoside 3-L-fucosyltransferase, FUTIV, FucT-IV), FUC-TIV, Fucosyltransferase IV(Fuc-TIV, Fucosyltransferase 4, FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, CD15, alpha-(1, 3)-fucosyltransferase 4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase

Anti-FUT4 Polyclonal Antibody is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-FUT4 Polyclonal Antibody

Anti-FUT4 Polyclonal Antibody

😃Good
Catalog No. TMAB-00722Alias SSEA-1, SSEA1, LeX, Galactoside 3-L-fucosyltransferase, FUTIV, FucT-IV), FUC-TIV, Fucosyltransferase IV(Fuc-TIV, Fucosyltransferase 4, FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, CD15, alpha-(1, 3)-fucosyltransferase 4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
Anti-FUT4 Polyclonal Antibody is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-FUT4 Polyclonal Antibody is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasSSEA-1, SSEA1, LeX, Galactoside 3-L-fucosyltransferase, FUTIV, FucT-IV), FUC-TIV, Fucosyltransferase IV(Fuc-TIV, Fucosyltransferase 4, FCT3A, ELFT, ELAM-1 ligand fucosyltransferase, EC:2.4.1.152, CD15, alpha-(1, 3)-fucosyltransferase 4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FUT4) Polyclonal Antibody, Unconjugated (TMAB-00722) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Overlay histogram showing HL 60 cells stained with TMAB-00722 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (TMAB-00722,1 μg/1x10^6 cells) for 30 min at 22°C. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (Brillant blue line) at 1/200 dilution for 30 min at 22°C. Isotype control antibody was rabbit IgG (polyclonal, Orange line) (1 μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
3. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FUT4/FITC Conjugated antibody (TMAB-00722-FITC)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature.
4. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-FUT4 (TMAB-00722)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-FUT4 (TMAB-00722) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 63 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FUT4
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
CD markers,Surface molecules,Adhesion,Surface Molecules,Endothelial Markers,Human Lineage Negative,Tumor Associated,Golgi
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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