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NHC-diphosphate

Name: NHC-diphosphate
Brand: TargetMol
SKU: T36880
Rating: 4.5 (1 reviews)

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Catalog No. T36880Cas No. 39023-73-9

NHC-diphosphate, a phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) in its diphosphate form[1], serves as a potent antiviral agent. As a pyrimidine ribonucleoside, NHC effectively counters the replication of Venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV), and hepatitis C virus (HCV)[1].

NHC-diphosphate

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Catalog No. T36880Cas No. 39023-73-9

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$315	Inquiry	Inquiry
5 mg	$664	Inquiry	Inquiry
10 mg	$995	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	NHC-diphosphate, a phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) in its diphosphate form[1], serves as a potent antiviral agent. As a pyrimidine ribonucleoside, NHC effectively counters the replication of Venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV), and hepatitis C virus (HCV)[1].
In vitro	Huh-7 cells were exposed to (10-50 μM; 4 h) either NHC or a McGuigan phosphoramidate prodrug of NHC. Subsequent analysis of intracellular levels of these compounds and their phosphorylated derivatives was performed using LC-MS/MS. The analysis revealed the presence of modest concentrations of NHC-monophosphate (MP) and NHC-diphosphate, with NHC-triphosphate being the predominant metabolite.[1].

Chemical Properties

Molecular Weight	419.18
Formula	C₉H₁₅N₃O₁₂P₂
Cas No.	39023-73-9
Smiles	ON=c1ccn([C@@H]2O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]2O)c(=O)[nH]1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 160 mg/mL (381.7 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	2.3856 mL	11.9281 mL	23.8561 mL	119.2805 mL
5 mM	0.4771 mL	2.3856 mL	4.7712 mL	23.8561 mL
10 mM	0.2386 mL	1.1928 mL	2.3856 mL	11.9281 mL
20 mM	0.1193 mL	0.5964 mL	1.1928 mL	5.9640 mL
50 mM	0.0477 mL	0.2386 mL	0.4771 mL	2.3856 mL
100 mM	0.0239 mL	0.1193 mL	0.2386 mL	1.1928 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc