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GUSB Protein, E. coli, Recombinant (His)

(Synonyms: β-glucuronidase, uidA, GUS, Beta-glucuronidase) Copy Product Info

Synonyms: β-glucuronidase, uidA, GUS, Beta-glucuronidase

Catalog No. TMPJ-00787 Copy Product Info
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
GUSB Protein, E. coli, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
20 µg$1987-10 days7-10 days
50 µg$3907-10 days7-10 days
100 µg$6267-10 days7-10 days
200 µg$9877-10 days7-10 days
500 µg$1,9007-10 days7-10 days
1 mg$2,7307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Appearance:Liquid
Color:Transparent
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Product Introduction

Bioactivity
Bioactivity
Measured by its ability to hydrolyze the substrate Phenolphthalein Glucuronide to Phenolphthalein and D-glucuronic acid. The specific activity is 19,660,754 U/g.
Description
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Species
E. coli
Expression System
E. coli
TagN-6xHis
Accession NumberP05804
Amino AcidMet1-Gln603
ConstructionMet1-Gln603
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationSupplied as a 0.2 μm filtered solution of PBS, pH 7.4.
Synonymsβ-glucuronidase, uidA, GUS, Beta-glucuronidase
Research Background
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Chemical Properties
Molecular Weight72 KDa (reducing condition)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: GUSB Protein, E. coli, Recombinant (His) chemical structure | GUSB Protein, E. coli, Recombinant (His) molecular weight