Shopping Cart

Your shopping cart is currently empty

PF-00835231

Name: PF-00835231
Brand: TargetMol
SKU: T9458
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

🥰Excellent

Catalog No. T9458Cas No. 870153-29-0

PF-00835231 is a CoV-2 cysteine 3C-like protease (3CLpro) inhibitor, with IC50s of 0.27 nM and 4 nM for SARS CoV-2 and SARS CoV-1 3CLpro, respectively. PF-00835231 is the active compound of the first anti-3CLpro regimen in clinical trials.

PF-00835231

Copy Product Info

🥰Excellent

Purity: 97.83%

Catalog No. T9458Cas No. 870153-29-0

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$52	In Stock	In Stock
2 mg	$74	In Stock	In Stock
5 mg	$123	In Stock	In Stock
10 mg	$197	In Stock	In Stock
25 mg	$357	In Stock	In Stock
50 mg	$536	In Stock	In Stock
100 mg	$788	In Stock	In Stock
200 mg	$1,090	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$135	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:97.83%

Appearance:Solid

Color:White

Resource Download

COA LCMS HPLC HNMR

Product Introduction

PF-00835231 AI Summary

PF-00835231 exhibits potent antiviral properties, particularly as an inhibitor of the 3CL protease of SARS-CoV-2 with an IC50 of 0.27 nM. It also effectively inhibits the 3CL protease of SARS coronavirus 1 with an IC50 of 4.0 nM and the protease of Human coronavirus 229E with an IC50 of 4.0 nM. For antiviral activity against infected cells, it demonstrates selective inhibition against Human coronavirus 229E in human MRC5 cells (EC50 of 9.0 nM) and against SARS-CoV-2 in Vero E6 cells (EC50 of 230.0 nM). The compound shows a relatively high EC50 of 4800.0 nM and 1100.0 nM against SARS COV-1 in monkey Vero 76 cells and low cytotoxicity (CC50 of 452,000.0 nM) in African green monkey Vero 76 cells, indicating a favorable therapeutic index. PF-00835231's diverse antiviral effects extend to other coronaviruses and human proteases such as Cathepsin L and B, with IC50 values of 146.0 nM and 1300.0 nM, respectively, demonstrating its broad-spectrum potential against viral pathogens..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	PF-00835231 is a CoV-2 cysteine 3C-like protease (3CLpro) inhibitor, with IC50s of 0.27 nM and 4 nM for SARS CoV-2 and SARS CoV-1 3CLpro, respectively. PF-00835231 is the active compound of the first anti-3CLpro regimen in clinical trials.
Targets&IC50	3CLpro (SARS CoV-2):4 nM, 3CLpro (SARS CoV-1):0.27 nM

Chemical Properties

Molecular Weight	472.53
Formula	C₂₄H₃₂N₄O₆
Cas No.	870153-29-0
Smiles	COc1cccc2[nH]c(cc12)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C[C@@H]1CCNC1=O)C(=O)CO
Relative Density.	1.264 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (529.07 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (10.58 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1163 mL	10.5813 mL	21.1627 mL	105.8134 mL
5 mM	0.4233 mL	2.1163 mL	4.2325 mL	21.1627 mL
10 mM	0.2116 mL	1.0581 mL	2.1163 mL	10.5813 mL
20 mM	0.1058 mL	0.5291 mL	1.0581 mL	5.2907 mL
50 mM	0.0423 mL	0.2116 mL	0.4233 mL	2.1163 mL
100 mM	0.0212 mL	0.1058 mL	0.2116 mL	1.0581 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc