Isoangustone A is a natural product that inhibits PI3K, MKK4, and MKK7 through ATP competition, suppresses the expression of G1 phase regulatory proteins, inhibits the phosphorylation of Akt, GSK-3β, and JNK1/2, and inhibits the proliferation of human melanoma cells and tumour growth in xenograft mouse models.

Method:
A cell proliferation assay was performed using the SK-MEL-28 cell line. Cells were treated with Isoangustone A at concentrations of 10 μM and 20 μM for 48 and 72 hours to evaluate its effect on cell proliferation.

Result:
Isoangustone A significantly inhibited the proliferation of SK-MEL-28 cells in a dose- and time-dependent manner. [1]

Method:
A SK-MEL-28 xenograft tumor model was established using male Balb/c nude (nu/nu) mice. Mice were administered Isoangustone A via daily intraperitoneal injection at doses of 2 or 10 mg/kg for 35 consecutive days to evaluate its antitumor effects.

Result:
Isoangustone A significantly reduced tumor weight compared to the control group. It also markedly decreased the expression of proliferating cell nuclear antigen (PCNA) and reduced the phosphorylation levels of Akt. [1]