This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
4-iodo-SAHA
Catalog No. T21749 CAS
1219807-87-0
4-Iodo-SAHA (1k), an orally active inhibitor of both class I and class II histone deacetylase (HDAC), exhibits EC50 values of 1.1, 0.95, 0.12, 0.24, 0.85, and 1.3 μM for Skbr3, HT29, U937, JA16, and HL60 cell lines, respectively. This compound holds potential for cancer research purposes [1].
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
4-Iodo-SAHA (1k), an orally active inhibitor of both class I and class II histone deacetylase (HDAC), exhibits EC50 values of 1.1, 0.95, 0.12, 0.24, 0.85, and 1.3 μM for Skbr3, HT29, U937, JA16, and HL60 cell lines, respectively. This compound holds potential for cancer research purposes [1].
In vitro
4-Iodo-SAHA (1k) demonstrates inhibitory effects on several cell lines including Skbr3, HT29, U937, JA16, and HL60 at concentrations ranging from 0.1-100 μM over 48 hours, according to study findings. Particularly in SKBR3 cells, exposure to 2 μM of 4-Iodo-SAHA for periods between 6-24 hours results in increased levels of acetylated H4 and p21. A Cell Proliferation Assay reveals that the compound successfully inhibits the growth of SKBR3, HT29, U937, JA16, and HL60 cell lines with EC50 values of 1.1, 0.95, 0.12, 0.24, 0.85, and 1.3 μM, respectively, indicating its 10-fold increased potency against the U937 cell line when compared to SAHA. Western Blot Analysis further corroborates these findings by showing a time-dependent increase in histone H4 acetylation and p21/WAF1 cell cycle inhibitor in SKBR3 cells, outlining 4-Iodo-SAHA's efficacy as both an inhibitor of cell proliferation and a modulator of cellular mechanisms related to acetylation and cell cycle regulation.
In vivo
4-Iodo-SAHA (1k) administered orally at a dosage of 50 mg/kg five times a week for two weeks exhibited comparable toxicity levels to those observed in SAHA-treated and control mice, as demonstrated by similar body weights and hematological counts in 8-week-old fvb mice [1].
Molecular Weight
390.22
Formula
C14H19IN2O3
CAS No.
1219807-87-0
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dose Conversion
You can also refer to dose conversion for different animals.
More
Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculator
Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
bottom
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.