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Puromycin dihydrochloride

Puromycin dihydrochloride
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Puromycin dihydrochloride

Catalog No. T2219Cas No. 58-58-2
Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
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Pack SizePriceAvailabilityQuantity
10 mg$39In Stock
25 mg$70In Stock
50 mg$122In Stock
100 mg$197In Stock
1 mL x 10 mM (in DMSO)$43In Stock
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Product Introduction

Puromycin dihydrochloride (CL13900 dihydrochloride) is a cinnamamide adenosine antibiotic and an inhibitor of protein synthesis. Puromycin dihydrochloride inhibits protein synthesis by binding to RNA and has antitumor and antitrypanosomal activity.
In vitro
METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively.[1]
METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection.
RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. [2]
METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL).
RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells. [3]
In vivo
Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.
AliasCL13900 dihydrochloride, Puromycin 2HCl
Chemical Properties
Molecular Weight544.44
Cas No.58-58-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
H2O: 92 mg/mL (169 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 60 mg/mL (110.2 mM)
Solution Preparation Table
1 mM1.8367 mL9.1837 mL18.3675 mL91.8375 mL
5 mM0.3673 mL1.8367 mL3.6735 mL18.3675 mL
10 mM0.1837 mL0.9184 mL1.8367 mL9.1837 mL
20 mM0.0918 mL0.4592 mL0.9184 mL4.5919 mL
50 mM0.0367 mL0.1837 mL0.3673 mL1.8367 mL
100 mM0.0184 mL0.0918 mL0.1837 mL0.9184 mL


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