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Orotic acid

Name: Orotic acid
Brand: TargetMol
SKU: T0746
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0746Cas No. 65-86-1

Alias Vitamin B13, 6-Carboxyuracil

Orotic acid (Vitamin B13) is an intermediate product in pyrimidine metabolism.

Orotic acid

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Purity: 99.42%

Catalog No. T0746Alias Vitamin B13, 6-CarboxyuracilCas No. 65-86-1

Orotic acid (Vitamin B13) is an intermediate product in pyrimidine metabolism.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$38	In Stock	In Stock
100 mg	$50	In Stock	In Stock
200 mg	$58	In Stock	In Stock
500 mg	$93	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.42%

Appearance:Solid

Color:White

Resource Download

COA HPLC HNMR LCMS

Product Introduction

Bioactivity

Description	Orotic acid (Vitamin B13) is an intermediate product in pyrimidine metabolism.
In vitro	Orotic acid increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1) [1].
In vivo	Male Fischer 344 rats (130-150 g) to two-thirds PH in the absence or in the presence of Orotic acid (a 300-mg tablet of Orotic acid methyl ester implanted intraperitoneally at the time of two-thirds PH). treatment with Orotic acid resulted in a near-100% inhibition of RNR induced by two-thirds PH in rat liver, as monitored by enzyme activity and protein level [2]. Feeding 1% Orotic acid with diet decreased the phosphorylation of AMPK and increased the maturation of SREBP-1 and the expression of SREBP-responsive genes in the rat liver. Orotic acid-induced lipid accumulation was also completely inhibited by rapamycin. Mouse hepatocytes and mice were resistant to Orotic acid-induced lipogenesis because of little if any response in AMPK and downstream effectors [3].
Synonyms	Vitamin B13, 6-Carboxyuracil

Chemical Properties

Molecular Weight	156.1
Formula	C₅H₄N₂O₄
Cas No.	65-86-1
Smiles	OC(=O)C1=CC(=O)NC(=O)N1
Relative Density.	1.6814 g/cm3 (Estimated)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 51.5 mg/mL (329.92 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2.5 mg/mL (16.02 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.4061 mL	32.0307 mL	64.0615 mL	320.3075 mL
5 mM	1.2812 mL	6.4061 mL	12.8123 mL	64.0615 mL
10 mM	0.6406 mL	3.2031 mL	6.4061 mL	32.0307 mL
20 mM	0.3203 mL	1.6015 mL	3.2031 mL	16.0154 mL
50 mM	0.1281 mL	0.6406 mL	1.2812 mL	6.4061 mL
100 mM	0.0641 mL	0.3203 mL	0.6406 mL	3.2031 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc