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Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation. TIM-4/TIMD4 Protein, Human, Recombinant (aa 25-314, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 32.43 kDa and the accession number is Q96H15-1.

| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 5 μg | $51 | 7-10 days | |
| 10 μg | $80 | 7-10 days | |
| 20 μg | $129 | 7-10 days | |
| 50 μg | $248 | 7-10 days | |
| 100 μg | $418 | 7-10 days | |
| 200 μg | $757 | 7-10 days | |
| 500 μg | $1,670 | 7-10 days |
| Biological Activity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation. TIM-4/TIMD4 Protein, Human, Recombinant (aa 25-314, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 32.43 kDa and the accession number is Q96H15-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q96H15-1 |
| Synonyms | TIMD-4,TIMD4,TIM-4,TIM4 |
| Construction | Glu25-Gln314 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Molecular Weight | 32.43 kDa (predicted). Due to glycosylation, the protein migrates to 78-98 kDa based on Tris-Bis PAGE result. |
| Endotoxin | < 1 EU/μg by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
| Research Background | Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation. |

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