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Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody

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Catalog No. TMAB-01402

Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DNM1L (Ser616). Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody

Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody

😃Good
Catalog No. TMAB-01402
Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DNM1L (Ser616). Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DNM1L (Ser616). Anti-Phospho-DNM1L (Ser616) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,GuineaPig)
Verified Activity
1. Blank control: U2-OS.
Primary Antibody (green line): Rabbit Anti-phospho-DNM1L (Ser616) antibody (TMAB-01402)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-DRP1 (Ser616)) Polyclonal Antibody, Unconjugated (TMAB-01402) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytosol. Golgi apparatus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Mainly cytosolic. Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human DRP1 around the phosphorylation site of Ser616
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism,Mitochondrial,Mitochondrial,Apoptosis,Mitochondrial markers,Neurodegenerative disease,Parkinson's disease,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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