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SMAD4 Protein, Human, Recombinant (His)

Catalog No. TMPJ-01108
Synonyms: DPC4, SMAD 4, Deletion Target in Pancreatic Carcinoma 4, hSMAD4, MADH4, Mothers Against Decapentaplegic Homolog 4, Mothers Against DPP Homolog 4, SMAD Family Member 4, MAD Homolog 4, SMAD4

SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
SMAD4 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 116.00
50 μg 5 days $ 352.00
500 μg 5 days $ 1,900.00
1 mg 5 days $ 2,730.00
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Biological Description
Technical Params
Product Properties
Description SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Species Human
Expression System E. coli
Tag C-6His
Accession Number Q13485
Synonyms DPC4, SMAD 4, Deletion Target in Pancreatic Carcinoma 4, hSMAD4, MADH4, Mothers Against Decapentaplegic Homolog 4, Mothers Against DPP Homolog 4, SMAD Family Member 4, MAD Homolog 4, SMAD4
Amino Acid Met1-Asp552
Construction Recombinant Human Mothers Against Decapentaplegic Homolog 4 is produced by our E.coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus.
Protein Purity Greater than 80% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 62 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.0.
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Stability & Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

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Keywords

SMAD4 Protein, Human, Recombinant (His) DPC4 MADH-4 SMAD 4 Deletion Target in Pancreatic Carcinoma 4 MADH 4 DPC 4 hSMAD4 MADH4 Mothers Against Decapentaplegic Homolog 4 Mothers Against DPP Homolog 4 SMAD Family Member 4 DPC-4 MAD Homolog 4 SMAD-4 SMAD4 recombinant recombinant-proteins proteins protein

 

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