CTLA-4 Protein, Human, Recombinant (hFc)

Name: CTLA-4 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05002
Rating: 4.5 (1 reviews)

(Synonyms: IDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5) Copy Product Info

Synonyms: IDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5

Catalog No. TMPY-05002 Copy Product Info

CTLA-4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.7 kDa and the accession number is P16410-1.

Pack Size	Price	USA Stock	Global Stock
10 µg	$29	7-10 days	7-10 days
20 µg	$43	7-10 days	7-10 days
50 µg	$79	7-10 days	7-10 days
100 µg	$129	7-10 days	7-10 days
200 µg	$222	7-10 days	7-10 days
500 µg	$450	7-10 days	7-10 days
1 mg	$893	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Loaded Recombinant Human CTLA-4 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human B7-1 Protein, His Tag with an affinity constant of 5.331nM as determined in BLI assay (Routinely tested).
Description	CTLA-4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.7 kDa and the accession number is P16410-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P16410-1
Construction	A DNA sequence encoding the human CTLA4 (NP_005205.2) (Met1-Phe162) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Lys 36
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	IDDM12, GSE, GRD4, cytotoxic T-lymphocyte-associated protein 4, CTLA-4, CELIAC3, CD152, CD, ALPS5
Research Background	Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsCTLA4 / CD152 Immune Checkpoint ProteinsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

40.7 kDa (predicted); 46-55 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.