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Anti-ERK5 Antibody (6Y531) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (6Y531) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $154 | 7-10 days | |
50 μL | $271 | 7-10 days | |
100 μL | $488 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB Reactivity: Human,Mouse (predicted:Rat) |
Ig Type | IgG |
Clone | 6Y531 |
Reactivity | Human,Mouse (predicted:Rat) |
Verified Activity | 1. Sample: Testis (Mouse) Lysate at 40 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-ERK5 (TMAB-00638) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 110 kDa 2. Blocking buffer: 5% NFDM/TBST Primary dilution: 1:2000 Primary incubation condition: 2 hours at room temperature Secondary: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: MCF-7, 2: 293T, 3: NIH/3T3, 4: PC- 12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 88 kDa Observed MW: 115 kDa |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. Translocates to the nucleus upon activation. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | Recombinant Protein: human ERK5 protein, around C-terminal 200aa |
Antigen Species | Human |
Gene ID | 5598 |
Uniprot ID | |
Biology Area | Neural Signal Transduction,Kinases,MAPK Pathway,Other Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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