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Anti-ERK5 Antibody (6Y531)

😃Good
Catalog No. TMAB-00638

Anti-ERK5 Antibody (6Y531) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (6Y531) can be used in WB.

Anti-ERK5 Antibody (6Y531)

Anti-ERK5 Antibody (6Y531)

😃Good
Catalog No. TMAB-00638
Anti-ERK5 Antibody (6Y531) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (6Y531) can be used in WB.
Pack SizePriceAvailabilityQuantity
25 μL$154 7-10 days
50 μL$271 7-10 days
100 μL$488 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB

Reactivity: Human,Mouse (predicted:Rat)
Ig Type
IgG
Clone
6Y531
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Sample:
Testis (Mouse) Lysate at 40 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-ERK5 (TMAB-00638) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 110 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: 293T, 3: NIH/3T3, 4: PC- 12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 88 kDa
Observed MW: 115 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Translocates to the nucleus upon activation.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
Recombinant Protein: human ERK5 protein, around C-terminal 200aa
Antigen Species
Human
Gene ID
5598
Uniprot ID
Biology Area
Neural Signal Transduction,Kinases,MAPK Pathway,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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