Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

HBV-IN-4

Name: HBV-IN-4
Brand: TargetMol
SKU: T36747
Rating: 4.5 (1 reviews)

Copy Product Info

😃Good

Catalog No. T36747Cas No. 2305897-84-9

HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].

HBV-IN-4

Copy Product Info

😃Good

Catalog No. T36747Cas No. 2305897-84-9

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$297	6-8 weeks	6-8 weeks
25 mg	$916	6-8 weeks	6-8 weeks
50 mg	$1,190	6-8 weeks	6-8 weeks
100 mg	$1,860	6-8 weeks	6-8 weeks

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Resource Download

Product Introduction

Bioactivity

Description	HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].
Targets&IC50	HBV DNA replication:14 nM (IC50)
In vitro	HBV-IN-4 (compound 19f; 0-1 μM; 8 days) effectively inhibits relaxed circular [rc] and single-stranded [ss] HBV DNA in a dose-dependent manner in HepG2.2.15 cells. It also decreases capsid-associated DNAs and promotes genome-free capsids, particularly those with accelerated migration phenotype[1].
In vivo	HBV-IN-4 (Compound 19f; 50-150 mg/kg; oral administration; twice a day; for 4 weeks; Balb/c male mice) achieves a 2.67 log viral load reduction in the AAV-HBV/mouse model[1]. It exhibits favorable drug characteristics, including low plasma clearance (CL=4.1 mL/min/kg), excellent drug exposure (AUC0-t=49,744 h·ng/L), T1/2 (2.15 hours), and oral bioavailability (F=60.4%) with 20 mg/kg oral administration in mice. HBV-IN-4 also shows good liver exposure distribution[1].

Chemical Properties

Molecular Weight	479.89
Formula	C₂₄H₁₉ClFN₅O₃
Cas No.	2305897-84-9
Smiles	OCC(O)CNc1nc(F)c(cc1Cl)-c1nn(Cc2ccc(cc2)C#N)c(=O)c2ccccc12

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (208.38 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (6.88 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0838 mL	10.4191 mL	20.8381 mL	104.1905 mL
5 mM	0.4168 mL	2.0838 mL	4.1676 mL	20.8381 mL
10 mM	0.2084 mL	1.0419 mL	2.0838 mL	10.4191 mL
20 mM	0.1042 mL	0.5210 mL	1.0419 mL	5.2095 mL
50 mM	0.0417 mL	0.2084 mL	0.4168 mL	2.0838 mL
100 mM	0.0208 mL	0.1042 mL	0.2084 mL	1.0419 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc