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HBV-IN-4

Catalog No. T36747 CAS 2305897-84-9

HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].

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HBV-IN-4, CAS 2305897-84-9

Pack Size	Availability	Price/USD
5 mg	6-8 weeks	$ 297.00
25 mg	6-8 weeks	$ 916.00
50 mg	6-8 weeks	$ 1,190.00
100 mg	6-8 weeks	$ 1,860.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].
Targets&IC50	HBV DNA replication:14 nM (IC50)
In vitro	HBV-IN-4 (compound 19f; 0-1 μM; 8 days) effectively inhibits different forms (relaxed circular [rc] and single-stranded [ss] HBV DNA) of the Hepatitis B virus in a dose-dependent fashion in HepG2.2.15 cells. Additionally, this treatment dose-dependently decreases capsid-associated DNAs and promotes the generation of genome-free capsids, notably those exhibiting a phenotype of accelerated migration[1].
In vivo	HBV-IN-4 (Compound 19f; 50-150 mg/kg; oral administration; twice a day; for 4 weeks; Balb/c male mice) treatment achieves 2.67 log viral load reduction in AAV-HBV/mouse model[1].HBV-IN-4 (compound 19f) exhibits favorable drug characteristics with low plasma clearance (CL=4.1 mL/min/kg), excellent drug exposure (AUC0-t=49 744 h?ng/L), T1/2 (2.15 hours) and oral bioavailability (F=60.4%) using 20 mg/kg oral administration in mice. HBV-IN-4 also shows good distribution in liver exposure[1].

Molecular Weight	479.89
Formula	C24H19ClFN5O3
CAS No.	2305897-84-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (208.38 mM), Sonication is recommended.

References and Literature

1. Wuhong Chen, et al. Discovery of Phthalazinone Derivatives as Novel Hepatitis B Virus Capsid Inhibitors. J Med Chem. 2020 Jul 21.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

HBV-IN-4 2305897-84-9 HBV IN 4 HBVIN4 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.