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Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody
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Catalog No. TMAB-01410
Alias PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, p-ERK5 (Ser496), p-ERK5 (S496), OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular Signal Regulated Kinase 5, ERK5 (p-Ser496), ERK5 (p-S496), ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK-1, BMK1, BMK 1 kinase, Big MAP kinase 1
Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK5 (Ser496). Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody
Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody
😃Good
Catalog No. TMAB-01410Alias PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, p-ERK5 (Ser496), p-ERK5 (S496), OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular Signal Regulated Kinase 5, ERK5 (p-Ser496), ERK5 (p-S496), ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK-1, BMK1, BMK 1 kinase, Big MAP kinase 1
Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK5 (Ser496). Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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| Description | Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK5 (Ser496). Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, p-ERK5 (Ser496), p-ERK5 (S496), OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular Signal Regulated Kinase 5, ERK5 (p-Ser496), ERK5 (p-S496), ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK-1, BMK1, BMK 1 kinase, Big MAP kinase 1 |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: Hcclm3 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-ERK5 (Ser496) (TMAB-01410) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 115 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-ERK5 (Ser496)) Polyclonal Antibody, Unconjugated (TMAB-01410) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-ERK5 (Ser496)) polyclonal Antibody, Unconjugated (TMAB-01410) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Translocates to the nucleus upon activation. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human ERK5 around the phosphorylation site of Ser496 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Neural Signal Transduction,Kinases,MAPK Pathway,Other Kinases |
| Molecular Weight | Theoretical: 90 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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