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Harringtonine

Name: Harringtonine
Brand: TargetMol
SKU: T8286
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8286Cas No. 26833-85-2

Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities.

Harringtonine

🥰Excellent

Purity: 98.94%

Catalog No. T8286Cas No. 26833-85-2

Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities.

Pack Size	Price	Availability
1 mg	$37	In Stock
5 mg	$79	In Stock
10 mg	$119	In Stock
25 mg	$198	In Stock
50 mg	$296	In Stock
1 mL x 10 mM (in DMSO)	$92	In Stock

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Purity:98.94%

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Product Introduction

Bioactivity

Description	Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities.
Targets&IC50	CHIKV:0.24 μM (EC50), HSV1:0.1081-0.1584 μM, HeLa cells:4 μM, BHK-21 cells:0.24 μM (EC50), HL-60 cells:0.047 μM
In vitro	Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit, blocking aminoacyl-tRNA binding and peptide bond formation [1]. It displays potent inhibition of Chikungunya virus infection (EC50: 0.24 μM) and could inhibit other alphaviruses [2]. Additionally, Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations [3].
In vivo	METHODS: To study in vivo activity, Harringtonine (0.5-4 mg/mouse) was injected retroorbital into C57BL/6 mice. RESULTS: Within 5 min, Harringtonine completely depleted multimers in all organs except testis and brain, and it took up to 30 min to significantly reduce the number of multimers due to the blood-brain and blood-testis barriers. [3]

Chemical Properties

Molecular Weight	531.59
Formula	C₂₈H₃₇NO₉
Cas No.	26833-85-2
Smiles	COC(=O)C[C@](O)(CCC(C)(C)O)C(=O)O[C@H]1[C@H]2c3cc4OCOc4cc3CCN3CCC[C@]23C=C1OC
Relative Density.	1.35 g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (103.46 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.76 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8811 mL	9.4057 mL	18.8115 mL	94.0575 mL
5 mM	0.3762 mL	1.8811 mL	3.7623 mL	18.8115 mL
10 mM	0.1881 mL	0.9406 mL	1.8811 mL	9.4057 mL
20 mM	0.0941 mL	0.4703 mL	0.9406 mL	4.7029 mL
50 mM	0.0376 mL	0.1881 mL	0.3762 mL	1.8811 mL
100 mM	0.0188 mL	0.0941 mL	0.1881 mL	0.9406 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc