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4-Di-1-ASP
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Catalog No. T78450Cas No. 959-81-9
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
4-Di-1-ASP
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Purity: 99.80%
Catalog No. T78450Cas No. 959-81-9
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 mg | $37 | In Stock | In Stock | |
| 200 mg | $54 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.80%
Appearance:Solid
Color:Purple
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | 4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm. |
| In vitro | Instructions (The following is a recommended protocol; actual procedures may be adjusted according to specific experimental needs)[1]: Dissolve the 4-Di-1-ASP in distilled water to prepare a 2 mM stock solution. Place the cell sections in a 35°C incubator, add the working dye solution to achieve a final concentration of 1 μM, and incubate for 10 minutes. After staining, mount the sections on a microscope slide, cover with a coverslip, and observe using a confocal fluorescence microscope. The optimal excitation and emission wavelengths are 475 nm and 606 nm, respectively. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 366.24 |
| Formula | C16H19IN2 |
| Cas No. | 959-81-9 |
| Smiles | [I-].C(=CC1=CC=[N+](C=C1)C)C2=CC=C(C=C2)N(C)C |
| Storage | keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 40 mg/mL (109.22 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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