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4-Di-1-ASP

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Catalog No. T78450Cas No. 959-81-9

4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.

4-Di-1-ASP

4-Di-1-ASP

😃Good
Purity: 99.80%
Catalog No. T78450Cas No. 959-81-9
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
100 mg$37In StockIn Stock
200 mg$54-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:99.80%
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COA HNMR LCMS

Product Introduction

Bioactivity
Description
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
In vitro
Instructions (The following is a recommended protocol; actual procedures may be adjusted according to specific experimental needs)[1]:
Dissolve the 4-Di-1-ASP in distilled water to prepare a 2 mM stock solution.
Place the cell sections in a 35°C incubator, add the working dye solution to achieve a final concentration of 1 μM, and incubate for 10 minutes.
After staining, mount the sections on a microscope slide, cover with a coverslip, and observe using a confocal fluorescence microscope. The optimal excitation and emission wavelengths are 475 nm and 606 nm, respectively.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Chemical Properties
Molecular Weight366.24
FormulaC16H19IN2
Cas No.959-81-9
Smiles[I-].C(=CC1=CC=[N+](C=C1)C)C2=CC=C(C=C2)N(C)C
Storage & Solubility Information
Storagekeep away from moisture,keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 40 mg/mL (109.22 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.7304 mL13.6522 mL27.3045 mL136.5225 mL
5 mM0.5461 mL2.7304 mL5.4609 mL27.3045 mL
10 mM0.2730 mL1.3652 mL2.7304 mL13.6522 mL
20 mM0.1365 mL0.6826 mL1.3652 mL6.8261 mL
50 mM0.0546 mL0.2730 mL0.5461 mL2.7304 mL
100 mM0.0273 mL0.1365 mL0.2730 mL1.3652 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
The above example illustrates how to use "In Vivo Formulation Calculator" and does not represent a recommended formulation for any specific compound. Please select an appropriate dissolution method based on your experimental animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
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%
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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