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4-Di-1-ASP
🥰Excellent
Catalog No. T78450Cas No. 959-81-9
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
4-Di-1-ASP
🥰Excellent
Purity: 99.80%
Catalog No. T78450Cas No. 959-81-9
4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 mg | $37 | In Stock | |
| 200 mg | $54 | In Stock |
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Purity:99.80%
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Product Introduction
Bioactivity
Chemical Properties
| Description | 4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex/λem = 475/606 nm. |
| In vitro | Instructions (The following is a recommended protocol; actual procedures may be adjusted according to specific experimental needs)[1]: Dissolve the 4-Di-1-ASP in distilled water to prepare a 2 mM stock solution. Place the cell sections in a 35°C incubator, add the working dye solution to achieve a final concentration of 1 μM, and incubate for 10 minutes. After staining, mount the sections on a microscope slide, cover with a coverslip, and observe using a confocal fluorescence microscope. The optimal excitation and emission wavelengths are 475 nm and 606 nm, respectively. |
| Molecular Weight | 366.24 |
| Formula | C16H19IN2 |
| Cas No. | 959-81-9 |
| Smiles | [I-].C(=CC1=CC=[N+](C=C1)C)C2=CC=C(C=C2)N(C)C |
Storage & Solubility Information
| Storage | keep away from moisture,keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 40 mg/mL (109.22 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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