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Anti-NCOA4 Polyclonal Antibody

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Catalog No. TMAB-01206

Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NCOA4 Polyclonal Antibody

Anti-NCOA4 Polyclonal Antibody

😃Good
Catalog No. TMAB-01206
Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Pig,Cow,Horse,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NCOA4) Polyclonal Antibody, Unconjugated (TMAB-01206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: HT1080 (Human) Cell Lysate at 30 μg
Lane 2: HepG2 (Human) Cell Lysate at 30 μg
Lane 3: U937 (Human) Cell Lysate at 30 μg
Primary:
Anti-NCOA4 (TMAB-01206) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 85 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NCOA4
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Co-activators/co-repressors,Co-activators/co-repressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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