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Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat,Pig,Cow,Horse,Sheep) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NCOA4) Polyclonal Antibody, Unconjugated (TMAB-01206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: HT1080 (Human) Cell Lysate at 30 μg Lane 2: HepG2 (Human) Cell Lysate at 30 μg Lane 3: U937 (Human) Cell Lysate at 30 μg Primary: Anti-NCOA4 (TMAB-01206) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 85 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009] |
Immunogen | KLH conjugated synthetic peptide: human NCOA4 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Co-activators/co-repressors,Co-activators/co-repressors |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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