PTK9 Protein, Human, Recombinant

Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein that belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 700.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 700.00

Description

Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	Q12792-4
Synonyms	MGC41876, twinfilin actin binding protein 1, A6, MGC23788, PTK9
Construction	A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 94 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 29 kDa

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4, 10% glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein that belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.