FGF-2 Protein, Human, Recombinant

(Synonyms: HBGF-2, fibroblast growth factor 2 (basic), FGFB, FGF-2, BFGF) Copy Product Info

Synonyms: HBGF-2, fibroblast growth factor 2 (basic), FGFB, FGF-2, BFGF

Catalog No. TMPY-00749 Copy Product Info

Purity: 98.7%

FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.

Pack Size	Price	USA Stock	Global Stock
5 µg	$106	-	In Stock
10 µg	$173	In Stock	In Stock
20 µg	$243	-	In Stock
50 µg	$386	-	In Stock
100 µg	$543	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.01-0.1 ng/mL.
Description	FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P09038-4
Construction	A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro143-Ser288) was expressed with an additional Met at the N-terminus. Predicted N terminal: Met
Protein Purity	≥ 95 % as determined by SDS-PAGE
Endotoxin	< 10 EU/mg of the protein.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.15 mg/mL.
Synonyms	HBGF-2, fibroblast growth factor 2 (basic), FGFB, FGF-2, BFGF
Research Background	Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.bFGF/FGF2 Protein & Antibody Products

Chemical Properties

Molecular Weight

16.5 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.