Antibody Type: Mouse Monoclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat

IgG

5Z89

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nucleus staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nucleus staining.
5. Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Heart tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Kidney tissue lysates
Lane 7: Rat Heart tissue lysates
Lane 8: Human HeLa cell lysates
Lane 9: Human Jurkat cell lysates
Lane 10: Human Raji cell lysates
Lane 11: Human 293T cell lysates
Lane 12: Human MCF-7 cell lysates
Primary: Anti-COX4I1 (Mitochondrial Loading Control) (TMAB-00473) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
6. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (Human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (COX4I1 (Mitochondrial Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-00473) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Monoclonal

Recombinant Protein: human COX4

Human

1327

Integration of energy metabolism,COX IV - Mitochondrial,Integration of energy,Mitochondrial markers,Complex IV,Mitochondrial,Mitochondria