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Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Alias | Histone H4K12-acetylated, HIST1H4J, HIST1H4I, HIST1H4H, HIST1H4F, HIST1H4E, HIST1H4D, HIST1H4C, HIST1H4B H4/I, HIST1H4A, HIST1, H4K12ac, H4FN, H4FM, H4FK, H4FJ, H4FI, H4FH, H4FG, H4FE, H4FD, H4FC, H4FB, H4FA, H4F2, H4/N, H4/M, H4/K, H4/J, H4/H, H4/G, H4/E, H4/D, H4/C, H4/B, H4/A, H4, FO108, Ac-Histone H4 (Lys12), Ac-Histone H4 (K12), Ac-H4K12, Acetyl-Histone H4 (K12) |
Ig Type | IgG |
Clone | 8Z904 |
Reactivity | Human (predicted:Mouse,Rat) |
Verified Activity | 1. Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:200 Primary incubation condition: 1 hour at room temperature Secondary: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-00036 2. Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary dilution: 1:100 Primary incubation condition: 4°C overnight Secondary: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for TMAB-00036 3. Blocking buffer: 5% NFDM/TBST Primary dilution: 1:2000 Primary incubation condition: 2 hours at room temperature Secondary: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 11 kDa Observed MW: 11 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-1:100; IF: 0 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Nucleus. Chromosome. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Antigen Affinity purification |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
Biology Area | Acetylated,ChIP antibodies |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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