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Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904)

😃Good
Catalog No. TMAB-00036
Alias Histone H4K12-acetylated, HIST1H4J, HIST1H4I, HIST1H4H, HIST1H4F, HIST1H4E, HIST1H4D, HIST1H4C, HIST1H4B H4/I, HIST1H4A, HIST1, H4K12ac, H4FN, H4FM, H4FK, H4FJ, H4FI, H4FH, H4FG, H4FE, H4FD, H4FC, H4FB, H4FA, H4F2, H4/N, H4/M, H4/K, H4/J, H4/H, H4/G, H4/E, H4/D, H4/C, H4/B, H4/A, H4, FO108, Ac-Histone H4 (Lys12), Ac-Histone H4 (K12), Ac-H4K12, Acetyl-Histone H4 (K12)

Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904)

Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904)

😃Good
Catalog No. TMAB-00036Alias Histone H4K12-acetylated, HIST1H4J, HIST1H4I, HIST1H4H, HIST1H4F, HIST1H4E, HIST1H4D, HIST1H4C, HIST1H4B H4/I, HIST1H4A, HIST1, H4K12ac, H4FN, H4FM, H4FK, H4FJ, H4FI, H4FH, H4FG, H4FE, H4FD, H4FC, H4FB, H4FA, H4F2, H4/N, H4/M, H4/K, H4/J, H4/H, H4/G, H4/E, H4/D, H4/C, H4/B, H4/A, H4, FO108, Ac-Histone H4 (Lys12), Ac-Histone H4 (K12), Ac-H4K12, Acetyl-Histone H4 (K12)
Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4897-10 days
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Product Introduction

Bioactivity
Description
Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
AliasHistone H4K12-acetylated, HIST1H4J, HIST1H4I, HIST1H4H, HIST1H4F, HIST1H4E, HIST1H4D, HIST1H4C, HIST1H4B H4/I, HIST1H4A, HIST1, H4K12ac, H4FN, H4FM, H4FK, H4FJ, H4FI, H4FH, H4FG, H4FE, H4FD, H4FC, H4FB, H4FA, H4F2, H4/N, H4/M, H4/K, H4/J, H4/H, H4/G, H4/E, H4/D, H4/C, H4/B, H4/A, H4, FO108, Ac-Histone H4 (Lys12), Ac-Histone H4 (K12), Ac-H4K12, Acetyl-Histone H4 (K12)
Ig Type
IgG
Clone
8Z904
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:200
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00036
2. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary dilution: 1:100
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-00036
3. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-1:2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-1:100; IF: 0
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus. Chromosome.
ConstructionHybridoma Monoclonal Antibody
PurificationAntigen Affinity purification
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008]
Antigen Details
Biology Area
Acetylated,ChIP antibodies
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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