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Thymidine

Thymidine
Thymidine (DThyd) is a cell synchronizing agent and a specific precursor of deoxyribonucleic acid. Thymidine inhibits DNA synthesis and can cause cell cycle arrest in the G1/S phase.
Catalog No. TWP2911Cas No. 50-89-5
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Thymidine

Catalog No. TWP2911Cas No. 50-89-5

Thymidine (DThyd) is a cell synchronizing agent and a specific precursor of deoxyribonucleic acid. Thymidine inhibits DNA synthesis and can cause cell cycle arrest in the G1/S phase.
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Pack SizePriceAvailabilityQuantity
500 mg$45In Stock
1 mL x 10 mM (in DMSO)$50In Stock
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Product Introduction

Bioactivity
Description
Thymidine (DThyd) is a cell synchronizing agent and a specific precursor of deoxyribonucleic acid. Thymidine inhibits DNA synthesis and can cause cell cycle arrest in the G1/S phase.
In vitro
METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Thymidine (300-1200 µM) and H2O2 (1 mM) for 0.5 h. DNA damage was detected by comet assay.
RESULTS: For the control and Thymidine alone groups, the samples appeared almost circular due to the absence of DNA damage. After exposure to 1.0 mM H2O2 for 0.5 h, a clear comet was observed, indicating DNA damage in this group. However, there was a decreasing trend in the extent of DNA damage in the three combination groups as the concentration of Thymidine pretreatment increased. [1]
METHODS: Jurkat, MOLT4 and NALM6 cells were treated with Thymidine (2 mM) for 18 h.
RESULTS: Thymidine arrests the cell cycle at the G1/S boundary. [2]
In vivo
METHODS: To investigate the effects of folate analogs on superantigen-reactive peripheral T cells in vivo, Thymidine (500 mg/kg) was injected intraperitoneally into MTX- or tomudex-administered mice twice daily for ten days.
RESULTS: Both MTX and tomudex blocked the expansion and accelerated the peripheral clearance of Vβ8-selective T cells. Thymidine administration completely eliminated this effect, suggesting that inhibition of glycoside synthase (TS), but not other folate-dependent enzymes, is the predominant mechanism. [3]
Alias5-Methyldeoxyuridine, Deoxyribothymidine, DThyd, NSC 21548, Thymidin
Chemical Properties
Molecular Weight242.23
FormulaC10H14N2O5
Cas No.50-89-5
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Saline: 25 mg/mL (103.21 mM)
Ethanol: Soluble
DMSO: 50 mg/mL (206.42 mM)
Pyridine, Methanol, etc.: Soluble
Solution Preparation Table
Saline/DMSO
1mg5mg10mg50mg
1 mM4.1283 mL20.6415 mL41.2831 mL206.4154 mL
5 mM0.8257 mL4.1283 mL8.2566 mL41.2831 mL
10 mM0.4128 mL2.0642 mL4.1283 mL20.6415 mL
20 mM0.2064 mL1.0321 mL2.0642 mL10.3208 mL
50 mM0.0826 mL0.4128 mL0.8257 mL4.1283 mL
100 mM0.0413 mL0.2064 mL0.4128 mL2.0642 mL

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