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IRAK-1-4 Inhibitor I

Name: IRAK-1-4 Inhibitor I
Brand: TargetMol
SKU: T2457
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2457Cas No. 509093-47-4

Alias IRAK-1/4 Inhibitor I, IRAK-1/4 Inhibitor

IRAK-1-4 Inhibitor I is a dual inhibitor targeting IRAK1 and IRAK4.

IRAK-1-4 Inhibitor I

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Purity: 99.89%

Catalog No. T2457Alias IRAK-1/4 Inhibitor I, IRAK-1/4 InhibitorCas No. 509093-47-4

IRAK-1-4 Inhibitor I is a dual inhibitor targeting IRAK1 and IRAK4.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$37	In Stock	In Stock
5 mg	$72	In Stock	In Stock
10 mg	$112	In Stock	In Stock
25 mg	$235	In Stock	In Stock
50 mg	$396	In Stock	In Stock
100 mg	$569	In Stock	In Stock
500 mg	$1,190	-	In Stock
1 mL x 10 mM (in DMSO)	$72	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.89%

Appearance:Solid

Color:White to Yellow

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COA

Product Introduction

Bioactivity

Description	IRAK-1-4 Inhibitor I is a dual inhibitor targeting IRAK1 and IRAK4.
Targets&IC50	IRAK1:0.2 μM, IRAK4:0.3 μM
In vitro	IRAK-1-4 Inhibitor I exhibits an IC50 greater than 10 μM against a panel of 27 kinases, including homologous kinases Lck and pp60SRC. It shows no cytotoxicity in a 72-hour proliferation assay in HeLa cells (ED50>30 μM). Significant inhibition of IRAK-1 is achieved with an IC50 of 0.3 μM[1]. IRAK-1/4 Inhibitor eliminates LPS-induced increases in Bcl10, NF-κB, and IL-8, with a 73% reduction in Bcl10 (from 5.18±0.22 to 2.36±0.08 ng/mL) and a 60% reduction in IL-8 (from 2.64±0.31 to 1.14±0.08 ng/mL)[2].
Kinase Assay	Kinase Assays: Cell-free kinase assays are done in quadruplicate with 1 μM ATP to determine the IC50 values of AV-951 against a variety of recombinant receptor and nonreceptor tyrosine kinases including VEGFR1, VEGFR2, VEGFR3, c-Kit, PDGFRβ, Flt-3 and FGFR1.
Cell Research	IRAK-1-4 Inhibitor I is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays[2].
Synonyms	IRAK-1/4 Inhibitor I, IRAK-1/4 Inhibitor

Chemical Properties

Molecular Weight	395.41
Formula	C₂₀H₂₁N₅O₄
Cas No.	509093-47-4
Smiles	[O-][N+](=O)c1cccc(c1)C(=O)Nc1nc2ccccc2n1CCN1CCOCC1
Relative Density.	1.40 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 7.88 mg/mL (19.93 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5290 mL	12.6451 mL	25.2902 mL	126.4510 mL
5 mM	0.5058 mL	2.5290 mL	5.0580 mL	25.2902 mL
10 mM	0.2529 mL	1.2645 mL	2.5290 mL	12.6451 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc