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P4HB Protein, Human, Recombinant (His)

(Synonyms: prolyl 4-hydroxylase, β polypeptide, prolyl 4-hydroxylase, beta polypeptide, PROHB, PO4HB, PO4DB, PHDB, PDIA1, PDI, P4Hβ, P4Hbeta, GIT, ERBA2L, DSI) Copy Product Info

Synonyms: prolyl 4-hydroxylase, β polypeptide, prolyl 4-hydroxylase, beta polypeptide, PROHB, PO4HB, PO4DB, PHDB, PDIA1, PDI, P4Hβ, P4Hbeta, GIT, ERBA2L, DSI

Catalog No. TMPY-01012 Copy Product Info
Purity: 97.2%
P4HB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is A0A024R8S5.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$48-In Stock
10 μg$757-10 days7-10 days
20 μg$1187-10 days7-10 days
50 μg$2297-10 days7-10 days
100 μg$386-In Stock
200 μg$6607-10 days7-10 days
500 μg$1,3307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:97.2%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 7.5 A650/min/mg
Description
P4HB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is A0A024R8S5.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP07237
ConstructionA DNA sequence encoding the human PH4B (NP_000909.2) corresponding to amino acid (Met 1-Lys 505) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Asp 18
Protein Purity
> 95 % as determined by SDS-PAGE
P4HB Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonymsprolyl 4-hydroxylase, β polypeptide, prolyl 4-hydroxylase, beta polypeptide, PROHB, PO4HB, PO4DB, PHDB, PDIA1, PDI, P4Hβ, P4Hbeta, GIT, ERBA2L, DSI
Research Background
Chemical Properties
Molecular Weight56.4 kDa (predicted); 60 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: P4HB Protein, Human, Recombinant (His) chemical structure | P4HB Protein, Human, Recombinant (His) molecular weight