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Timosaponin AIII

Name: Timosaponin AIII
Brand: TargetMol
SKU: T3395
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T3395Cas No. 41059-79-4

Alias Timosaponin A3, Filiferin B, AneMarsaponin A3

Timosaponin AIII (Timosaponin A3) induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM). The Timosaponin AIII cellular response is mediated via inhibition of mTORC1 and induction of ER stress (IC50: 2.5 μM, BT474 cells; 6 μM, MDAMB231). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.

Timosaponin AIII

🥰Excellent

Purity: 99.61%

Catalog No. T3395Alias Timosaponin A3, Filiferin B, AneMarsaponin A3Cas No. 41059-79-4

Pack Size	Price	Availability
1 mg	$30	In Stock
5 mg	$64	In Stock
10 mg	$113	In Stock
25 mg	$239	In Stock
50 mg	$343	In Stock
100 mg	$515	In Stock
200 mg	$747	In Stock
1 mL x 10 mM (in DMSO)	$97	In Stock

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Batch Information

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Purity:99.61%

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Timosaponin AIII (Timosaponin A3) induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM). The Timosaponin AIII cellular response is mediated via inhibition of mTORC1 and induction of ER stress (IC50: 2.5 μM, BT474 cells; 6 μM, MDAMB231). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.
Targets&IC50	mTORC1(BT474 cells):2.5 μM, mTORC1(MDAMB231):6 μM
Synonyms	Timosaponin A3, Filiferin B, AneMarsaponin A3

Chemical Properties

Molecular Weight	740.92
Formula	C₃₉H₆₄O₁₃
Cas No.	41059-79-4
Smiles	C[C@@]12[C@@]3([C@](C[C@]1([C@]4([C@](CC2)([C@]5(C)[C@](CC4)(C[C@@H](O[C@H]6[C@H](O[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H](O)[C@@H](O)[C@@H](CO)O6)CC5)[H])[H])[H])[H])(O[C@@]8([C@H]3C)CC[C@H](C)CO8)[H])[H]
Relative Density.	1.366 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (67.48 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3497 mL	6.7484 mL	13.4967 mL	67.4837 mL
5 mM	0.2699 mL	1.3497 mL	2.6993 mL	13.4967 mL
10 mM	0.1350 mL	0.6748 mL	1.3497 mL	6.7484 mL
20 mM	0.0675 mL	0.3374 mL	0.6748 mL	3.3742 mL
50 mM	0.0270 mL	0.1350 mL	0.2699 mL	1.3497 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

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