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Timosaponin AIII

Name: Timosaponin AIII
Brand: TargetMol
SKU: T3395
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Timosaponin A3, Filiferin B, AneMarsaponin A3) Copy Product Info

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Synonyms: Timosaponin A3, Filiferin B, AneMarsaponin A3

Catalog No. T3395 Copy Product Info

Purity: 99.61%

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Timosaponin AIII (Timosaponin A3) induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM). The Timosaponin AIII cellular response is mediated via inhibition of mTORC1 and induction of ER stress (IC50: 2.5 μM, BT474 cells; 6 μM, MDAMB231). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.

Cas No. 41059-79-4

Pack Size	Price	USA Stock	Global Stock
1 mg	$30	In Stock	In Stock
5 mg	$64	In Stock	In Stock
10 mg	$113	In Stock	In Stock
25 mg	$239	In Stock	In Stock
50 mg	$343	In Stock	In Stock
100 mg	$515	-	In Stock
200 mg	$747	-	In Stock
1 mL x 10 mM (in DMSO)	$97	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.61%

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Timosaponin AIII (Timosaponin A3) induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM). The Timosaponin AIII cellular response is mediated via inhibition of mTORC1 and induction of ER stress (IC50: 2.5 μM, BT474 cells; 6 μM, MDAMB231). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.
Targets & IC50	mTORC1(MDAMB231):6 μM, mTORC1(BT474 cells):2.5 μM
Synonyms	Timosaponin A3, Filiferin B, AneMarsaponin A3

Chemical Properties

Molecular Weight	740.92
Formula	C₃₉H₆₄O₁₃
Cas No.	41059-79-4
Smiles	C[C@@]12[C@@]3([C@](C[C@]1([C@]4([C@](CC2)([C@]5(C)[C@](CC4)(C[C@@H](O[C@H]6[C@H](O[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H](O)[C@@H](O)[C@@H](CO)O6)CC5)[H])[H])[H])[H])(O[C@@]8([C@H]3C)CC[C@H](C)CO8)[H])[H]
Relative Density.	1.366 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (337.42 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3497 mL	6.7484 mL	13.4967 mL	67.4837 mL
5 mM	0.2699 mL	1.3497 mL	2.6993 mL	13.4967 mL
10 mM	0.1350 mL	0.6748 mL	1.3497 mL	6.7484 mL
20 mM	0.0675 mL	0.3374 mL	0.6748 mL	3.3742 mL
50 mM	0.0270 mL	0.1350 mL	0.2699 mL	1.3497 mL
100 mM	0.0135 mL	0.0675 mL	0.1350 mL	0.6748 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc