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Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 mg | $42 | - | In Stock | |
| 100 mg | $54 | - | In Stock | |
| 1 mL x 10 mM (in DMSO) | $45 | - | In Stock |
| Description | Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al. |
| In vitro | Instructions a. Solution preparation: Dissolve lumogallion in DMSO or 0.1 M acetate buffer (pH 5.2) and prepare a 10mM storage solution for later use. Note: The storage solution needs to be stored at -20℃ or -80℃. Please avoid repeated freezing and thawing. The diluted working solution should be prepared and used as soon as possible. b. Operation steps: 1. Incubate the sample with 10 μM lumogallion in the dark for 60 min. 2. Wash the mixed sample twice with acetate buffer, 15 minutes each time. 3. Observe under a laser confocal microscope, with an excitation wavelength of 488 nm and an emission wavelength of 520 nm. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | 4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid |
| Molecular Weight | 344.73 |
| Formula | C12H9ClN2O6S |
| Cas No. | 4386-25-8 |
| Smiles | Oc1ccc(\N=N\c2cc(Cl)cc(c2O)S(O)(=O)=O)c(O)c1 |
| Relative Density. | 1.75g/cm3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 50 mg/mL (145.04 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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