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Lumogallion

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Catalog No. T20984Cas No. 4386-25-8
Alias 4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid

Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al.

Lumogallion

Lumogallion

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Purity: 99.65%
Catalog No. T20984Alias 4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acidCas No. 4386-25-8
Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 mg$42-In Stock
100 mg$54-In Stock
1 mL x 10 mM (in DMSO)$45-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.65%
Appearance:Solid
Color:Red
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Product Introduction

Bioactivity
Description
Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al.
In vitro
Instructions
a. Solution preparation: Dissolve lumogallion in DMSO or 0.1 M acetate buffer (pH 5.2) and prepare a 10mM storage solution for later use.
Note: The storage solution needs to be stored at -20℃ or -80℃. Please avoid repeated freezing and thawing. The diluted working solution should be prepared and used as soon as possible.
b. Operation steps:
1. Incubate the sample with 10 μM lumogallion in the dark for 60 min.
2. Wash the mixed sample twice with acetate buffer, 15 minutes each time.
3. Observe under a laser confocal microscope, with an excitation wavelength of 488 nm and an emission wavelength of 520 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid
Chemical Properties
Molecular Weight344.73
FormulaC12H9ClN2O6S
Cas No.4386-25-8
SmilesOc1ccc(\N=N\c2cc(Cl)cc(c2O)S(O)(=O)=O)c(O)c1
Relative Density.1.75g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 50 mg/mL (145.04 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.9008 mL14.5041 mL29.0082 mL145.0410 mL
5 mM0.5802 mL2.9008 mL5.8016 mL29.0082 mL
10 mM0.2901 mL1.4504 mL2.9008 mL14.5041 mL
20 mM0.1450 mL0.7252 mL1.4504 mL7.2521 mL
50 mM0.0580 mL0.2901 mL0.5802 mL2.9008 mL
100 mM0.0290 mL0.1450 mL0.2901 mL1.4504 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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