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Galegine hydrochloride

Name: Galegine hydrochloride
Brand: TargetMol
SKU: T35532
Rating: 4.5 (1 reviews)

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Catalog No. T35532Cas No. 2368870-39-5

Galegine hydrochloride, a guanidine derivative derived from G. officinalis, plays a role in inducing weight loss in mice and has contributed to the development of biguanides, including metformin and phenformin. This compound stimulates AMPK activation in 3T3-L1 adipocytes, L6 myotubes, H4IIE rat hepatoma, and HEK293 human kidney cell lines. Additionally, galegine hydrochloride exhibits antibacterial properties, particularly demonstrating a minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains[1][2].

Galegine hydrochloride

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Catalog No. T35532Cas No. 2368870-39-5

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	Inquiry	6-8 weeks	6-8 weeks
50 mg	Inquiry	6-8 weeks	6-8 weeks
100 mg	Inquiry	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	Galegine hydrochloride, a guanidine derivative derived from G. officinalis, plays a role in inducing weight loss in mice and has contributed to the development of biguanides, including metformin and phenformin. This compound stimulates AMPK activation in 3T3-L1 adipocytes, L6 myotubes, H4IIE rat hepatoma, and HEK293 human kidney cell lines. Additionally, galegine hydrochloride exhibits antibacterial properties, particularly demonstrating a minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains[1][2].
In vitro	Pre-treatment with Galegine hydrochloride (10 μM-3 mM; 5 h) enhances insulin-independent glucose absorption in 3T3-L1 adipocytes concentration-dependently, without impacting cell health. Similarly, incubation (1 μM-1 mM, 5 h) boosts glucose intake in L6 myotubes without affecting viability[1]. Galegine hydrochloride (0.3-300 μM; 24 hours) slightly decreases basal glycerol release and significantly reduces isoprenaline-induced glycerol release in 3T3-L1 adipocytes. In H4IIE rat hepatoma cells, Galegine hydrochloride (10 or 300 μM) up to 6 hours progressively activates AMPK, reaching peak activation at 360 min and maintaining twofold activation at 24 hours with 300 μM, showing a stronger effect at 300 μM than at 10 μM. A one-hour incubation triggers concentration-dependent AMPK activation in both 3T3L-1 adipocytes and L-6 myotubes, as well as in a human kidney cell line (HEK293)[1].
In vivo	Galegine hydrochloride (63 mg/kg; feed; daily for 11 days) significantly reduces body weight[1].

Chemical Properties

Molecular Weight	163.65
Formula	C₆H₁₄ClN₃
Cas No.	2368870-39-5
Smiles	Cl.CC(C)=CCNC(N)=N

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 5.2 mg/mL (31.78 mM), Sonication is recommended.

H2O: 43.33 mg/mL (264.77 mM), Sonication is recommended.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	6.1106 mL	30.5530 mL	61.1060 mL	305.5301 mL
5 mM	1.2221 mL	6.1106 mL	12.2212 mL	61.1060 mL
10 mM	0.6111 mL	3.0553 mL	6.1106 mL	30.5530 mL
20 mM	0.3055 mL	1.5277 mL	3.0553 mL	15.2765 mL

H2O

	1mg	5mg	10mg	50mg
50 mM	0.1222 mL	0.6111 mL	1.2221 mL	6.1106 mL
100 mM	0.0611 mL	0.3055 mL	0.6111 mL	3.0553 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc