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NOD-IN-1

Name: NOD-IN-1
Brand: TargetMol
SKU: T3587
Price: 44 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3587Cas No. 132819-92-2

Alias Compound 4

NOD-IN-1 (Compound 4) is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors.

NOD-IN-1

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Purity: 99.93%

Catalog No. T3587Alias Compound 4Cas No. 132819-92-2

NOD-IN-1 (Compound 4) is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$44	In Stock	In Stock
5 mg	$98	In Stock	In Stock
10 mg	$155	In Stock	In Stock
25 mg	$297	In Stock	In Stock
50 mg	$429	In Stock	In Stock
100 mg	$626	-	In Stock
1 mL x 10 mM (in DMSO)	$98	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.93%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	NOD-IN-1 (Compound 4) is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors.
Targets&IC50	NOD1:5.74 μM, NOD2:6.45 μM
In vitro	NOD-IN-1, also known as compound 4, is a potent mixed inhibitor of NOD1 and NOD2, demonstrating balanced inhibitory effects on both targets within the low micromolar range. With IC50 values of 5.74 μM for NOD1 and 6.45 μM for NOD2, NOD-IN-1 is highlighted as the most effective compound in its series, showcasing significative inhibition capabilities in the lower micromolar spectrum. Comparative analysis reveals that NOD-IN-1 is seven times less effective than Noditinib-1 at inhibiting NOD1 and lacks selective inhibitory activity towards either NOD1 or NOD2, contrasting with Noditinib-1. Nonetheless, NOD-IN-1 maintains balanced dual inhibitory activities on both targets at concentrations below 10 μM[1].
Cell Research	NOD-IN-1 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. An MTS assay in which the proliferation rates of HEK-Blue NOD1 cells are measured in the presence of Noditinib-1 and of the synthesized potential NOD1 inhibitor NOD-IN-1 is employed to screen these compounds for potential cytotoxicity. Cells are treated for 24 h with the compound of interest at concentrations of up to 25 μM. Comparison of the resulting metabolic activities with that of the untreated control showed that all compounds are well tolerated by HEK-Blue NOD1 cells, since their residual metabolic activities do not fall below 80% at the maximum concentration tested[1].
Synonyms	Compound 4

Chemical Properties

Molecular Weight	343.4
Formula	C₁₈H₁₇NO₄S
Cas No.	132819-92-2
Smiles	CCOC(=O)c1cc2ccccc2n1S(=O)(=O)c1ccc(C)cc1
Relative Density.	1.27 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (160.16 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.82 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9121 mL	14.5603 mL	29.1206 mL	145.6028 mL
5 mM	0.5824 mL	2.9121 mL	5.8241 mL	29.1206 mL
10 mM	0.2912 mL	1.4560 mL	2.9121 mL	14.5603 mL
20 mM	0.1456 mL	0.7280 mL	1.4560 mL	7.2801 mL
50 mM	0.0582 mL	0.2912 mL	0.5824 mL	2.9121 mL
100 mM	0.0291 mL	0.1456 mL	0.2912 mL	1.4560 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc