Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10^6 IU/mg.

IL-4 Protein, Rat, Recombinant is expressed in E. coli. The predicted molecular weight is 14.0 kDa and the accession number is P20096.

Rat

E. coli

Lymphocyte stimulatory factor 1,Interleukin-4,IL4,BSF-1,B-cell stimulatory factor 1,B-cell IGG differentiation factor,B-cell growth factor 1

HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

23-147 aa

> 95% as determined by SDS-PAGE and HPLC.

Reconstitute the lyophilized protein in sterile deionized water containing 0.1% BSA. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.