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FtsZ-IN-4

Catalog No. T61737

FtsZ-IN-4, an orally active inhibitor of FtsZ (filamenting temperature-sensitive mutant Z), demonstrates remarkable antibacterial activity and favorable pharmaceutical properties. With low cytotoxicity (CC 50 <20 μg/mL) [1], FtsZ-IN-4 exhibits promising potential for therapeutic applications.

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FtsZ-IN-4, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 1,520.00
50 mg	10-14 weeks	$ 1,980.00
100 mg	10-14 weeks	$ 2,500.00

Bulk Inquiry

This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	FtsZ-IN-4, an orally active inhibitor of FtsZ (filamenting temperature-sensitive mutant Z), demonstrates remarkable antibacterial activity and favorable pharmaceutical properties. With low cytotoxicity (CC 50 >20 μg/mL) [1], FtsZ-IN-4 exhibits promising potential for therapeutic applications.
In vitro	FtsZ-IN-4, identified as compound 30, exhibits potent antibacterial effects against B. subtilis and S. aureus, with minimum inhibition concentrations (MIC) of 0.008-0.25 μg/mL, according to standards [1]. This compound demonstrates rapid bactericidal activity, achieving significant reductions in bacterial population within 3 hours at concentrations of 0.064 μg/mL or 0.5 μg/mL, with minimum bactericidal concentration (MBC) to MIC ratios of ≤4, indicating efficacy within Clinical and Laboratory Standards Institute (CLSI) guidelines [1]. Additionally, at concentrations exceeding 20 μg/mL over 72 hours, FtsZ-IN-4 shows minimal cytotoxicity towards Vero cells, with a 50% cytotoxic concentration (CC50) >20 μg/mL [1]. It notably disrupts bacterial cell division, elongating B. subtilis ATCC9372 within 3 hours at 0.016 μg/mL and induces aberrant cell division leading to bacterial cell death [1]. Furthermore, FtsZ-IN-4 enhances SaFtsZ polymerization at 10 μg/mL within 0-15 minutes and inhibits SaFtsZ's GTPase activity in a dose-dependent manner within 30 minutes across concentrations of 0-35 μg/mL [1]. In cell proliferation assays, the compound significantly decreased populations of S. aureus ATCC25923 and B. subtilis ATCC9372 to below detectable limits within 3 hours at multiples of the MIC, establishing its effective bactericidal capabilities [1].
In vivo	FtsZ-IN-4 (compound 30) demonstrates moderate pharmacokinetic exposure (AUC (0-t) = 544.2 hng/mL) and an oral bioavailability (F) of 61.2% following a 5 mg/kg oral (p.o.) administration in mice [1]. Administered intravenously (i.v.) at a dose of 25 mg/kg, it shows significant in vivo efficacy, reducing bacterial burden in male ICR mice infected with S. aureus ATCC25923 to levels comparable with vancomycin, as evidenced by its pharmacokinetic profile which includes a half-life (T 1/2) of 0.28 hours and a peak concentration (Cmax) of 480.5 ng/mL following a 1 mg/kg dose, and enhanced exposure metrics (AUC (0-t) = 544.2 hng/mL) at a 5 mg/kg dose, confirming its effectiveness as an intraperitoneal injection [1].

Molecular Weight	387.81
Formula	C21H16ClF2NO2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Reconstitution Calculation

Molecular Weight Calculator

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An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
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The answer of 19.713 mg appears in the Mass box

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Calculator the dilution required to prepare a stock solution

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The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
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The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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Molecule Formula

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Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

FtsZ-IN-4 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.