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Anti-COX4I1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00472
Alias mitochondrial, Cytochrome c oxidase subunit IV isoform 1 (COX IV-1), Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, COX4I1, COX4
Anti-COX4I1 Polyclonal Antibody is a Rabbit antibody targeting COX4I1. Anti-COX4I1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-COX4I1 Polyclonal Antibody
Anti-COX4I1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00472Alias mitochondrial, Cytochrome c oxidase subunit IV isoform 1 (COX IV-1), Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, COX4I1, COX4
Anti-COX4I1 Polyclonal Antibody is a Rabbit antibody targeting COX4I1. Anti-COX4I1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-COX4I1 Polyclonal Antibody is a Rabbit antibody targeting COX4I1. Anti-COX4I1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | mitochondrial, Cytochrome c oxidase subunit IV isoform 1 (COX IV-1), Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, COX4I1, COX4 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated (TMAB-00472) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (TMAB-00472) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Heart tissue lysates Lane 3: Mouse Muscle tissue lysates Lane 4: Rat Kidney tissue lysates Lane 5: Rat Heart tissue lysates Lane 6: Rat Muscle tissue lysates Lane 7: Human HeLa cell lysates Lane 8: Human Jurkat cell lysates Lane 9: Human THP-1 cell lysates Lane 10: Human Raji cell lysates Lane 11: Human HepG2 cell lysates Lane 12: Human MCF-7 cell lysates Primary: Anti-COX4I1 (TMAB-00472) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 16 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion inner membrane. |
| Tissue Specificity | Ubiquitous. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human COX4I1 |
| Antigen Species | Human |
| Gene Name | COX4I1 |
| Gene ID | |
| Protein Name | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,COX IV - Mitochondrial,Integration of energy,Mitochondrial markers,Complex IV,Mitochondrial,Mitochondria |
| Function | This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. |
| Molecular Weight | Theoretical: 17 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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