Anti-COX4I1 Polyclonal Antibody is a Rabbit antibody targeting COX4I1. Anti-COX4I1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated (TMAB-00472) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (TMAB-00472) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Mouse Muscle tissue lysates
Lane 4: Rat Kidney tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Muscle tissue lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human THP-1 cell lysates
Lane 10: Human Raji cell lysates
Lane 11: Human HepG2 cell lysates
Lane 12: Human MCF-7 cell lysates
Primary: Anti-COX4I1 (TMAB-00472) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 16 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human COX4I1

Human

Integration of energy metabolism,COX IV - Mitochondrial,Integration of energy,Mitochondrial markers,Complex IV,Mitochondrial,Mitochondria