Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-EpCAM Polyclonal Antibody

😃Good
Catalog No. TMAB-00622

Anti-EpCAM Polyclonal Antibody is a Rabbit antibody targeting EpCAM. Anti-EpCAM Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-EpCAM Polyclonal Antibody

Anti-EpCAM Polyclonal Antibody

😃Good
Catalog No. TMAB-00622
Anti-EpCAM Polyclonal Antibody is a Rabbit antibody targeting EpCAM. Anti-EpCAM Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-EpCAM Polyclonal Antibody is a Rabbit antibody targeting EpCAM. Anti-EpCAM Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rabbit (predicted:Rat,Dog,Pig,Cow)
Verified Activity
1. Tissue/cell: mouse embryo tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EpCAM Polyclonal Antibody, Unconjugated (TMAB-00622) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EpCAM Polyclonal Antibody, Unconjugated (TMAB-00622) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (TMAB-00622) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (TMAB-00622) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationLateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EpCAM
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Epi / Endo-thelial,Tumor Associated
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-EpCAM Polyclonal Antibody | purchase Anti-EpCAM Polyclonal Antibody | Anti-EpCAM Polyclonal Antibody cost | order Anti-EpCAM Polyclonal Antibody