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Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His)

(Synonyms: Nucleoprotein, NP Protein) Copy Product Info

Synonyms: Nucleoprotein, NP Protein

Catalog No. TMPY-04688 Copy Product Info
Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 63.1 kDa and the accession number is B4UQF0.

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Purity:93%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 63.1 kDa and the accession number is B4UQF0.
Species
Influenza B
Expression System
Baculovirus Insect Cells
TagC-His
Accession NumberB4UQF0
ConstructionA DNA sequence encoding the Influenza B (B/Florida/4/2006) nucleoprotein (ACF54251.1) (Met1-Tyr560) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Met
Protein Purity
> 90 % as determined by SDS-PAGE.
Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 500 mM NaCl, 10% Glycerol, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsNucleoprotein, NP Protein
Research Background
Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Chemical Properties
Molecular Weight63.1 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His) chemical structure | Influenza B (B/Florida/4/2006) Nucleoprotein/NP Protein (His) molecular weight